3T6-Swiss albino Cells
General information
Description | The 3T6-Swiss albino cell line, established by G. Todaro and H. Green in 1963 from disaggregated Swiss mouse embryos, is well-known in vitro model for cell growth and differentiation. These cells are useful for studying extracellular matrix biology and tissue engineering because they secrete collagen and hyaluronic acid. 21% of 3T6-Swiss albino cells have an extra large chromosome with a terminal centromere and 21% have minute chromosomes, indicating an unstable karyotype in the stemline range (78-81), which makes them a great tool for studying chromosomal instability. The 3T6-Swiss Albino cells have also been used to study oncogenes and tumor suppressor genes. Other properties of the 3T6-Swiss Albino cell line are that they are non-tumorigenic and highly homogeneous. The 3T6-Swiss Albino cell line is useful for studying basic cellular biology, extracellular matrix biology, chromosomal instability, and cancer therapeutics and tissue engineering. |
---|---|
Organism | Mouse |
Tissue | Embryonic |
Applications | This cell line is an optimal choice for transfection. |
Synonyms | 3T6 Swiss Albino, Swiss 3T6, NIH 3T6, 3T6, GM05862 |
Characteristics
Age | Embryo |
---|---|
Morphology | Fibroblast-like |
Cell type | Fibroblast |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | 3T6-Swiss albino (Cytion catalog number 400104) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Tumorigenic | No |
---|---|
Viruses | Negative for ectromelia virus (mousepox). |
Virus susceptibility | Herpes simplex, Vaccinia, Pseudorabies, Vesicular Stomatitis (Indiana) |
Reverse transcriptase | Negative |
Products | Collagen, hyaluronic acid |
Ploidy status | Karyotyping results revealed an unstable range of 78-81. A significant portion (21%) of the cells contained a terminal centromere on a large chromosome, and another 21% comprised minuscule chromosomes. |
Handling
Culture Medium | Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:10 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 will result in a confluent monolayer within 5 days. |
Fluid renewal | Every 3 to 4 days |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|