SW620 Cells

CAD$545.10*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300466

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The SW-620 cell line, originating from the large intestine of a 51-year-old male with Dukes-C colorectal cancer, is a pivotal research model in colorectal cancer, especially for cancer biomarkers, chemotherapy, and the study of metastatic cancer cells. SW-620 cells are pivotal for studying cell apoptosis and the resistance mechanisms to anoikis, a form of programmed cell death crucial for preventing metastasis. Research utilizing the SW-620 colon cancer cells has delved into proteomic analysis to understand proteome changes under different conditions, such as hypoxia. Hypoxic SW620 cells exhibit specific proteome adaptations that contribute to chemotherapy resistance. SW620 colon cancer cells have been pivotal in evaluating natural compounds like curcumin and their impact on cancer cell viability. Studies have shown that curcumin inhibits cell viability in SW-620 cells. Moreover, the cell line aids in assessing the effects of chemotherapeutic agents and the potential for chemotherapy resistance, which is critical for advancing cancer treatment strategies. Exhibiting high tumorigenic and metastatic capabilities, SW-620 cells form solid tumors in vivo. The SW620 xenograft model, alongside the study of specific pathways like the catenin pathway and the role of transcription factors such as cdx2 in colonic adenocarcinoma cells, enriches our understanding of colorectal cancer's molecular underpinnings. In summary, SW-620 human colon adenocarcinoma cells are an invaluable resource in cancer research, offering a multifaceted approach to understanding colorectal cancer's complexities.
Organism	Human
Tissue	Colorectal
Disease	Adenocarcinoma
Metastatic site	Lymph node
Synonyms	SW620, SW 620, SW.620

Age	51 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth properties	Adherent

Citation	SW-620 (Cytion catalog number 300466)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0547

Tumorigenic	Yes, in athymic nude mice
Karyotype	Average number of chromosomes 48 (range, 46-52). Eighteen marker chromosomes. For a detailed description of the karyotype we refer to Melcher et al.

Culture Medium	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Fluid renewal	2 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300466-150925	Certificate of Analysis	05. Dec. 2025	300466
300466-322F	Certificate of Analysis	23. May. 2025	300466

SW620 Cells

Basic details about the SW-620 cancer cell line

Aspects

Specification

Genetic makeup of the colorectal cancer cell line SW620

Maintenance techniques

Quality assurance of SW620 cells

Certificate of Analysis (CoA)

Organism	Human
Tissue	Colon, grade IV, Dukes' type C
Disease	Adenocarcinoma

Organism	Human
Tissue	Colon
Disease	Adenocarcinoma, Grade IV, Dukes' type B.

Organism	Human
Tissue	Urinary bladder
Disease	Bladder carcinoma