LoVo Cell Line

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

CAD$545.10*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300266

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The LOVO cell line, derived from a grade IV Dukes' type C colon adenocarcinoma, is characterized by mutations in the adenomatous polyposis coli (APC) gene, Kirsten rat sarcoma viral oncogene homolog (KRAS), and tumor protein p53 (TP53). These genetic features are instrumental in studying the molecular basis of colorectal cancer progression, metastasis, and drug resistance mechanisms. LoVo cells serve as a critical model for screening anti-cancer compounds and by understanding how cancer cells like LoVo develop resistance, researchers can design more effective therapies. LoVo cells are also employed in molecular biology studies to explore signaling pathways that regulate cancer cell growth, survival, and metastasis. In the context of human colon cancer and colorectal cancer cell lines, LoVo cells offer insights into the mechanisms of tumor growth and the process of metastasis, particularly node metastasis, and the tumor microenvironment driving cancer progression. The use of LoVo colon cancer cells, especially in lovo xenograft models, allows researchers to study cancer cell dynamics and metastatic potential. Deep sequencing and gene expression analysis in LoVo cells have shed light into the specific genes and their roles in colorectal cancer cells. This research has has highlighted the importance of integrins, such as integrin β1, in cancer cell migration and invasion, and the regulation of key molecules like MMP2 in signaling pathways contributing to the understanding of cancer cell lines' invasive properties. LoVo cells, as a model system in colorectal cancer cell lines, play a pivotal role in advancing our understanding of the molecular aspects of cancer, from gene and protein expression to the intricacies of tumor growth and metastasis.
Organism	Human
Tissue	Colon, grade IV, Dukes' type C
Disease	Adenocarcinoma
Metastatic site	Left supraclavicular lymph node
Synonyms	LOVO

Age	56 years
Gender	Male
Morphology	Epithelial-like
Growth properties	Adherent

Citation	LoVo (Cytion catalog number 300266)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_0399

Antigen expression	HLA A11, B15, B17, Cw1, Cw3, blood type B
Isoenzymes	G6PD, B, PGM1, 2, PGM3, 1-2, 6PGD, A, ES-D, 1
Oncogenes	Myc +, myb + , ras +, fos +, p53 +, sis -, abl -, ros -, src -
Tumorigenic	Yes, in nude mice
Reverse transcriptase	Negative
Products	Carcinoembryonic antigen (CEA) 908 ng/106 cells/10 days
Mutational profile	LOVO cells carry a mutation in codon 13 of Kras gene: GGC(Wt Gly) >GAC(Asp)

Culture Medium	Ham's F12K Medium, w: 2.0 mM L-Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.5 g/L NaHCO3 (Cytion article number 820608a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	1 x 10⁴ cells/cm²
Fluid renewal	2 to 3 times per week
Post-Thaw Recovery	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300266-140825	Certificate of Analysis	22. Oct. 2025	300266

LoVo Cell Line

Insights on Lovo cells

Aspects

Documentation about LoVo cells

Genomics

Handling the LoVo cell line

Quality control

Certificate of Analysis (CoA)