NCI-H716 Cells

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 305079

安全資料表

產品說明書

分析證明書 (CoA)

說明	The NCI-H716 cell line is a human adenocarcinoma cell line derived from the colon. It was established from the metastatic site in the ascites of a 33-year-old Caucasian male. One of the defining features of the NCI-H716 cell line is its ability to express and secrete enteroendocrine hormones, notably glucagon-like peptide 1 (GLP-1), which makes it highly relevant in the study of gut hormone physiology and the enteroendocrine system. This aspect is crucial for diabetes research, especially in the context of investigating the hormonal regulation of insulin secretion and glucose homeostasis. These cells are adapted to grow as floating aggregates or in suspension culture, which is somewhat unusual for epithelial-derived cells. The ability to grow in suspension allows the study of cellular interactions and signaling pathways in a three-dimensional culture environment, which can mimic in vivo conditions more closely than traditional monolayer cultures. The NCI-H716 cell line has been utilized extensively to explore signal transduction pathways involved in the secretion of hormones, response to pharmacological agents, and the interaction between gut epithelial cells and the microbiota. Studies using this cell line have contributed significantly to understanding the pathophysiology of gastrointestinal diseases and the development of therapeutic strategies targeting the gut-brain axis. Furthermore, NCI-H716 cells are used to test therapeutic compounds for their potential effects on secretion and receptor response. Their unique hormonal profile also enables their use in pharmacodynamic studies and drug discovery related to metabolic disorders and obesity. Thus, NCI-H716 serves as a vital tool in translational medicine, bridging basic research and clinical applications in gastrointestinal and metabolic diseases.
生物體	Human
組織	Cecum
疾病	Cecum adenocarcinoma
轉移部位	Ascites
同義詞	NCI H716, NCI-H716, H-716, NCIH716

年齡	33 years
性別	Male
族裔	European
形態學	Epithelial
生長特性	Suspension, multicell aggregates and some adherent cells

引用	NCI-H716 (Cytion catalog number 305079)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_1581

培養基	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
營養補充品	Supplement the medium with 10% FBS
倍增時間	50 hours
傳代培養	Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10⁵ cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation.
播種密度	> 3 x 10⁵ cells/ml
流體更新	Add 1 ml of fresh medium daily, weekends may be omitted, and separate clusters by pipetting as required.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.