SW620 Cells

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 300466

安全資料表

產品說明書

分析證明書 (CoA)

說明	The SW-620 cell line, originating from the large intestine of a 51-year-old male with Dukes-C colorectal cancer, is a pivotal research model in colorectal cancer, especially for cancer biomarkers, chemotherapy, and the study of metastatic cancer cells. SW-620 cells are pivotal for studying cell apoptosis and the resistance mechanisms to anoikis, a form of programmed cell death crucial for preventing metastasis. Research utilizing the SW-620 colon cancer cells has delved into proteomic analysis to understand proteome changes under different conditions, such as hypoxia. Hypoxic SW620 cells exhibit specific proteome adaptations that contribute to chemotherapy resistance. SW620 colon cancer cells have been pivotal in evaluating natural compounds like curcumin and their impact on cancer cell viability. Studies have shown that curcumin inhibits cell viability in SW-620 cells. Moreover, the cell line aids in assessing the effects of chemotherapeutic agents and the potential for chemotherapy resistance, which is critical for advancing cancer treatment strategies. Exhibiting high tumorigenic and metastatic capabilities, SW-620 cells form solid tumors in vivo. The SW620 xenograft model, alongside the study of specific pathways like the catenin pathway and the role of transcription factors such as cdx2 in colonic adenocarcinoma cells, enriches our understanding of colorectal cancer's molecular underpinnings. In summary, SW-620 human colon adenocarcinoma cells are an invaluable resource in cancer research, offering a multifaceted approach to understanding colorectal cancer's complexities.
生物體	Human
組織	Colorectal
疾病	Adenocarcinoma
轉移部位	Lymph node
同義詞	SW620, SW 620, SW.620

年齡	51 years
性別	Male
族裔	Caucasian
形態學	Epithelial-like
生長特性	Adherent

引用	SW-620 (Cytion catalog number 300466)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_0547

致瘤性	Yes, in athymic nude mice
核型分析	Average number of chromosomes 48 (range, 46-52). Eighteen marker chromosomes. For a detailed description of the karyotype we refer to Melcher et al.

培養基	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
流體更新	2 times per week
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300466-322F	分析證明書	23. May. 2025	300466
300466-150925	分析證明書	05. Dec. 2025	300466

SW620 Cells

Basic details about the SW-620 cancer cell line

Aspects

Specification

Genetic makeup of the colorectal cancer cell line SW620

Maintenance techniques

Quality assurance of SW620 cells

分析證明書 (CoA)

生物體	Human
組織	Colon, grade IV, Dukes' type C
疾病	Adenocarcinoma

生物體	Human
組織	Colon
疾病	Adenocarcinoma, Grade IV, Dukes' type B.

生物體	Human
組織	Urinary bladder
疾病	Bladder carcinoma