SW-403 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 300350

安全資料表

產品說明書

分析證明書 (CoA)

說明	SW-403 is a human colorectal adenocarcinoma cell line derived from a poorly differentiated tumor. It has been widely used in research on colorectal cancer, particularly in studies investigating the effects of gastrointestinal hormones on tumor growth. Notably, SW-403 cells have been shown to respond to gastrin and pentagastrin, two gastrointestinal hormones, by increasing their proliferation. These hormones stimulate growth through the gastrin receptor, which is expressed in some colorectal cancers. In contrast, treatment with proglumide, a gastrin receptor antagonist, inhibits the growth of SW-403 cells both in vitro and in vivo, suggesting that gastrin may play a role in promoting tumor growth in this cell line. In addition to hormone studies, SW-403 cells have been used to investigate the effects of various chemotherapy agents, such as ciprofloxacin, on cancer cell proliferation and apoptosis. Ciprofloxacin has been shown to inhibit DNA synthesis in SW-403 cells and induce apoptosis in a dose-dependent manner. This process involves mitochondrial membrane breakdown, activation of caspases 3, 8, and 9, and upregulation of pro-apoptotic proteins like Bax. The ability of ciprofloxacin to trigger apoptosis in SW-403 cells suggests its potential as an adjunctive therapeutic agent in colorectal cancer treatment. Overall, SW-403 serves as a useful model for exploring the molecular mechanisms underlying colorectal cancer growth, hormone sensitivity, and chemotherapy-induced apoptosis. Its response to gastrointestinal hormones like gastrin and to chemotherapeutic agents highlights its relevance in both basic cancer biology and drug development research.
生物體	Human
組織	Colon
疾病	Adenocarcinoma
同義詞	SW403, SW 403

年齡	51 years
性別	Female
族裔	Caucasian
形態學	Epithelial-like
生長特性	Adherent

引用	SW-403 (Cytion catalog number 300350)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_0545

抗原表達	Colon antigen 3, positive. The cells are positive for keratin by immunoperoxidase staining. CSAp negative (CSAp-).
同工酶	G6PD, B, PGM1, 1, PGM3, 1-2, 6PGD, A, ES-D, 1, PEP-D, 1
致瘤性	Yes, in nude mice
逆轉錄酶	Negative
產品	Carcinoembryonic antigen (CEA) 155 ng/10 exp6 cells/10 days, keratin
突變譜	SW-403 cells carry a heterozygous Kras mutation in codon12: GGT>GTT

培養基	Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
流體更新	1 to 2 times per week
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300350-413	分析證明書	26. May. 2025	300350
300350-150925	分析證明書	05. Dec. 2025	300350

SW-403 Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)

生物體	Human
組織	Colon
疾病	Adenocarcinoma, Grade IV, Dukes' type B.

生物體	Human
組織	Urinary bladder
疾病	Bladder carcinoma