IHH-4 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 305448

安全資料表

產品說明書

分析證明書 (CoA)

說明	The IHH-4 cell line is derived from papillary thyroid carcinoma (PTC), the most prevalent form of thyroid cancer, which frequently exhibits aggressive characteristics including invasion and metastasis. IHH-4 has been utilized in numerous studies focused on elucidating the molecular mechanisms underpinning PTC progression. This cell line is particularly noted for its role in studies investigating epithelial-mesenchymal transition (EMT), a process that enhances the invasive potential of cancer cells. For example, it has been shown that IHH-4 cells, along with other PTC lines, express elevated levels of matrix metalloproteinase-9 (MMP-9), a protease that plays a critical role in degrading the extracellular matrix and facilitating tumor invasion and metastasis. Inhibition of MMP-9 in IHH-4 cells was found to reduce EMT markers and hinder cell migration and invasion. Research involving the IHH-4 cell line has also examined the role of transcription factors such as T cell factor 4 (TCF4) and long non-coding RNAs (lncRNAs) in PTC. Studies have highlighted that TCF4 is overexpressed in IHH-4 cells and can regulate the expression of the lncRNA HCP5, which in turn modulates several microRNAs related to tumor progression. Knockdown of TCF4 in IHH-4 cells was shown to decrease cell proliferation and invasion, suggesting that TCF4 is a pivotal regulator of oncogenic pathways in PTC. Overall, IHH-4 serves as a valuable model for studying molecular and cellular pathways related to thyroid cancer, particularly those that drive cancer cell invasion, metastasis, and resistance to therapies. The insights gained from research using IHH-4 contribute to the development of potential therapeutic strategies for combating aggressive thyroid cancers.
生物體	Human
組織	Thyroid gland
疾病	Thyroid gland papillary carcinoma
轉移部位	Left cervical lymph node
同義詞	IHH4

年齡	75 years
性別	Male
族裔	Japanese
形態學	Epithelial-like
生長特性	Adherent

引用	IHH-4 (Cytion catalog number 305448)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_2960
轉基因狀態	GMO-S1: This human thyroid papillary carcinoma cell line (IHH-4) contains undefined stable modifications consistent with tumor-derived immortalization. No infectious virus is produced. This classification applies only within Germany and may differ elsewhere.

突變譜	Mutation: AKT1, p.Glu17Lys (c.49G>A), heterozygous; Mutation: BRAF, p.Val600Glu (c.1799T>A), heterozygous; Mutation: CREBBP, p.Trp592Ter (c.1776G>A), heterozygous; Mutation: CRLF2, p.Trp255Ter (c.765G>A), heterozygous; Mutation: EP300, p.Arg1312Ter (c.3934C>T), heterozygous; Mutation: RAC1, p.Asp11Glu (c.33C>G), heterozygous; Mutation: TERT, c.1-124C>T (c.228C>T) (C228T), heterozygous

培養基	1 to 1 mixture of Dulbecco's modified Eagle's medium (Cytion article number 820300a) and RPMI1640 medium (Cytion article number 820700a)
營養補充品	Supplement the medium with 10% heat-inactivated FBS
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
305448-301025	分析證明書	05. Dec. 2025	305448

IHH-4 Cells

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品質控制與分子分析

分析證明書 (CoA)