CCD-1095Sk Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

產品編號： 300642

安全資料表

產品說明書

分析證明書 (CoA)

說明	CCD-1095Sk is a fibroblast cell line derived from the skin of a human male. It was established from a biopsy of uninvolved skin taken from a patient who had a squamous cell carcinoma. This cell line is utilized primarily in studies that explore the interactions between skin cells and cancerous cells, particularly how non-cancerous cells in the tumor microenvironment can influence tumor growth and progression. The CCD-1095Sk cell line is therefore valuable for cancer research, specifically for understanding the stromal aspects of skin cancer. The CCD-1095Sk cells exhibit a fibroblast morphology, characterized by a spindle-shaped, elongated form typical of connective tissue cells that produce extracellular matrix components essential for tissue repair and structural integrity. These cells are adherent, grow in monolayers, and are known for their robustness in various in vitro experimental conditions. They are used to model fibroblast behavior in normal skin and to examine changes in fibroblast activity under cancerous conditions, which can include the secretion of growth factors, cytokines, and matrix metalloproteinases. As such, they provide an invaluable tool for pharmacological studies and the development of therapeutic strategies targeting the tumor environment.
生物體	Human
組織	Skin
疾病	Ductal carcinoma
應用	3D cell culture
同義詞	CCD1095Sk

年齡	37 years
性別	Female
形態學	Fibroblast
生長特性	Adherent

引用	CCD-1095Sk (Cytion catalog number 300642)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	CVCL_2344

培養基	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
營養補充品	Supplement the medium with 10% FBS and 1% NEAA
解離試劑	Accutase
傳代培養	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
冷凍培養基	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

不孕症	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

批次編號	證書類型	日期	型號
300642-230725	分析證明書	18. Aug. 2025	300642

CCD-1095Sk Cells

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)