A2780-GFP

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USUSD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

價格不含稅，另加運費

cryovial

產品編號： 305676

安全資料表

產品說明書

分析證明書 (CoA)

說明	A2780-GFP is a genetically modified derivative of the human ovarian carcinoma cell line A2780, engineered to stably express green fluorescent protein (GFP). The parental A2780 cell line was established from an ovarian tumor in an adult patient and is widely used as a model for epithelial ovarian cancer, particularly in studies of chemotherapy response. It is known for its relative sensitivity to platinum-based agents such as cisplatin, making it a valuable system for investigating mechanisms of drug action and resistance. The GFP-expressing variant preserves these biological characteristics while incorporating a fluorescent reporter for enhanced experimental utility. The stable expression of GFP enables real-time visualization and quantitative analysis of A2780-GFP cells in a variety of experimental settings. Fluorescence-based detection allows researchers to monitor cell proliferation, morphology, migration, and viability in vitro, as well as to track tumor growth and dissemination in vivo. This is particularly advantageous in xenograft and metastasis models, where GFP facilitates discrimination of tumor cells from surrounding host tissue. The fluorescent signal is generally stable across passages, although expression levels may vary depending on the transduction method and clonal selection. A2780-GFP retains the core molecular and phenotypic features of the parental A2780 line, including pathways associated with DNA damage response, apoptosis, and chemotherapeutic sensitivity. As such, it is commonly used in high-content imaging assays, drug screening platforms, and co-culture systems where spatial and temporal resolution of tumor cell behavior is required. The addition of GFP significantly enhances the versatility of this model, supporting applications in ovarian cancer research, therapeutic evaluation, and studies of tumor cell dynamics.
生物體	Human
組織	Ovary
疾病	Ovarian endometrioid adenocarcinoma
轉移部位	Primary tumor site (ovary)
應用	Ovarian cancer imaging; GFP-based tumor tracking; in vivo fluorescence imaging; combination with parental A2780 for comparative studies; drug efficacy visualization

年齡	Age unspecified
性別	Female
族裔	African American
形態學	Epithelial-like
細胞類型	Epithelial cells
生長特性	Adherent

引用	A2780-GFP (Cytion catalog number 305676)
生物安全等級	1
NCBI_TaxID	9606
Cellosaurus 編號	Not assigned (A2780-GFP reporter derivative; parental A2780 CVCL_1099)
轉基因狀態	GMO-S1: This human A2780 ovarian cancer line contains a lentiviral GFP expression construct for fluorescence-based imaging. This classification applies only within Germany and may differ elsewhere.

抗原表達	GFP

培養基	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
營養補充品	Supplement the medium with 10% FBS
解離試劑	Accutase
拆股比例	1 to 5
播種密度	1 to 3 × 10⁴ cells/cm²
流體更新	2 to 3 times per week
冷凍培養基	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
細胞解凍與培養	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
培養環境	37°C, 5% CO₂, humidified atmosphere.
運送條款	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
儲存條件	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

批次編號	證書類型	日期	型號
305676-040326	分析證明書	08. Apr. 2026	305676

A2780-GFP

一般資訊

特徵

監管數據

生物分子資料

處理方式

品質控制與分子分析

分析證明書 (CoA)