MDA-MB-175-VII Cells

US$ 550,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 305825

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	MDA-MB-175-VII is a human breast cancer cell line originally derived from the pleural effusion of an adult female patient with infiltrating ductal mammary carcinoma. The cell line is part of a series established from metastatic breast tumors to provide viable, fibroblast-poor epithelial cultures. Specifically, MDA-MB-175 was isolated from six of eight thoracenteses performed on a patient who underwent mastectomy and exhibited recurrent malignant pleural effusions. The tumor cells were consistently viable and cultured successfully across samples, which provided a stable platform for in vitro studies of metastatic breast cancer biology. MDA-MB-175-VII cells are morphologically epithelial and have a modal chromosome number of approximately 49, reflecting an aneuploid karyotype. These cells exhibit relatively slow growth in vitro but have gained scientific interest due to their unique molecular features, including the expression of neuregulin-1 (NRG1) fusion transcripts. In particular, the NRG1-DOC4 fusion observed in this line leads to constitutive activation of the HER3/HER4 receptor pathway, promoting autocrine signaling and cell proliferation. This molecular characteristic has positioned MDA-MB-175-VII as a rare but critical model for studying autocrine HER-family receptor signaling and its pharmacological targeting in breast cancer . Further integration into large-scale datasets such as the Cancer Cell Line Encyclopedia (CCLE) has enabled deeper molecular profiling of MDA-MB-175-VII. These datasets include transcriptomic, mutational, and proteomic information that support the classification of the cell line within the luminal subtype of breast cancers, with modest sensitivity to agents targeting HER-family receptors and PI3K signaling pathways. As such, MDA-MB-175-VII serves as a valuable model for preclinical investigations of targeted therapies and the functional consequences of oncogenic gene fusions in breast cancer.
Organismo	Human
Tecido	Metastatic
Doença	Invasive breast carcinoma of no special type
Local da metástase	Pleural effusion
Sinônimos	MDA MB 175 VII, MDA-MB-175VII, MDAMB175VII, MDA-MB-175, MDAMB175, MDA-175, MDA175, MD Anderson-Metastatic Breast-175-VII

Idade	56 years
Gênero	Female
Etnia	African American
Morfologia	Epithelial
Tipo de célula	Epithelial
Propriedades de crescimento	Adherent

Referência	MDA-MB-175VII (Cytion catalog number 305825)
Nível de biossegurança	1
NCBI_TaxID	9606
Número de acesso do Cellosaurus	CVCL_1400

Isoenzimas	AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 PGM1, 2 PGM3, 1-2
Tumorigênico	Yes; Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.
Perfil mutacional	Mutation: Gene fusion, NRG1 + HGNC, TENM4, Name(s)=TENM4-NRG1, DOC4-NRG1, Note=In frame.
Cariótipo	Model number = 84; range = 82 to 89

Meio de cultura	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Suplementos	Supplement the medium with 10% FBS + Insulin (5 microgram/ml)
Reagente de dissociação	Accutase
Tempo de duplicação	112 hours
Renovação de fluidos	2 to 3 times per week
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.