HK-CRISPR-Pom121-mCherry Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

US$ 800,00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Número do produto: 300658

Ficha de dados de segurança

Ficha técnica do produto

Certificado de Análise (CoA)

Descrição	The HK-CRISPR-Pom121-mCherry cell line is a genetically engineered variant of HeLa Kyoto cells, designed for advanced cellular research. These cells have been modified using CRISPR-Cas9 technology to add a fluorescent mCherry tag to the Pom121 gene, a key component of the nuclear pore complex (NPC). This modification allows researchers to visualize Pom121 in real-time, aiding studies of nuclear dynamics and NPC functions. The mCherry tag, emitting red fluorescence, facilitates detailed examination of NPC behavior and nuclear-cytoplasmic transport using fluorescence microscopy. This cell line is a valuable tool for investigating nuclear pore proteins, gene expression regulation, cell cycle control, and signal transduction.
Organismo	Human
Tecido	Endocervix
Doença	Adenocarcinoma

Idade	30 years
Gênero	Female
Etnia	African American
Morfologia	Epithelial-like cells with mosaic stone shape
Propriedades de crescimento	Adherent

Referência	HK-CRISPR-Pom121-mCherry (Cytion catalog number 300658)
Nível de biossegurança	1
NCBI_TaxID	9606
Depositante	The Ellenberg Lab (EMBL)
Situação em relação aos OGMs	GMO-S1: This HeLa Kyoto line contains a CRISPR-mediated mCherry tag on Pom121 enabling visualization of nuclear pore membrane components. This classification applies only within Germany and may differ elsewhere.

Expressão de proteínas	Pom121, mCherry-tag

Meio de cultura	DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a)
Suplementos	Supplement the medium with 10% FBS
Reagente de dissociação	Accutase
Subcultura	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Meio de congelamento	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Descongelamento e cultura de células	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosfera de incubação	37°C, 5% CO₂, humidified atmosphere.
Condições de envio	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Condições de armazenamento	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Esterilidade	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Número do lote	Tipo de certificado	Data	Número de catálogo
300658-110624	Certificado de Análise	23. May. 2025	300658

HK-CRISPR-Pom121-mCherry Cells

Informações gerais

Características

Dados regulatórios

Dados biomoleculares

Manuseio

Controle de Qualidade e Análise Molecular

Certificado de Análise (CoA)