CHO-K1 Cells

545,10 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 603480

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	CHO-K1 cells are a subline derived from the CHO cell line, which was originally established in the early 1950s from a Chinese hamster ovary. CHO-K1 cells are widely utilized in the production of therapeutic monoclonal antibodies and other biopharmaceuticals. Their extensive use in biopharmaceutical protein production and vaccines is attributed to their eukaryotic nature, which allows for proper folding, assembly, and post-translational modifications such as glycosylation, which influences the stability, efficacy, and safety of the produced proteins. In the realm of recombinant protein production, the CHO-K1 cell line is used to express a wide array of proteins, including monoclonal antibodies, growth factors, cytokines, and enzymes. These proteins have applications in therapeutic treatments, diagnostic assays, and vaccine formulations. CHO-K1 cells exhibit a robust growth rate and are adaptable to various culture conditions, including suspension and adherent cultures, making them highly valuable for large-scale bioproduction processes. They possess a high level of genetic stability and are used for stable cell line development as they are capable of amplifying and expressing exogenous genes efficiently, which is critical for producing high yields of recombinant proteins. CHO-K1 chinese hamster cells can be easily transfected with a variety of vectors for gene expression, facillitating gene editing or knockdown. This flexibility allows researchers to introduce specific genes, silence genes, or even perform targeted gene editing using technologies like CRISPR-Cas9 in CHO-K1 host cells. In conclusion, the chinese hamster CHO-K1 cells and CHO cells are pivotal in biotechnological research and biopharmaceutical production, offering a versatile platform for the study of gene function and the large-scale production of recombinant proteins.
Organisme	Chinese hamster
Tissu	Ovary
Applications	This cell line is an optimal choice for toxicology, industrial biotechnology and bioproduction.
Synonymes	CHO K1, CHOK1, CHO cell clone K1, GM15452

Âge	Adult
Genre	Female
Morphologie	Epithelial-like
Propriétés de croissance	Monolayer, adherent

Référence	CHO-K1 (Cytion catalog number 603480)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	10029
Cellosaurus - Numéro d'enregistrement	CVCL_0214

Sensibilité aux virus	Vesicular stomatitis (Indiana), Getah virus Virus Resist: poliovirus 2, modoc virus, Button Willow virus
Transcriptase inverse	Negative
Caryotype	Chromosome Frequency Distribution 50 Cells: 2n = 22. Stemline number is hypodiploid

Milieu de culture	Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	Accutase
Temps de doublement	22 hours
Repiquage	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Densité de semis	1 x 10⁴ cells/cm² will yield in a confluent layer in about 6 days
Renouvellement des fluides	2 to 3 times per week
Rétablissement après le dégel	After thawing, plate the cells at 5 x 10⁴ cells/cm² and allow the cells to recover from the freezing process and to adhere for at least 24 hours.
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
603480-291124	Certificat d'analyse	18. Aug. 2025	603480

CHO-K1 Cells

Basic details about the CHO-K1 cell line

Properties of CHO-K1 cells

Documenation

Genetic characteristics of the chinese hamster ovary cell line CHO

Culturing guidelines

Quality control

Certificat d'analyse (CoA)

Organisme	Chinese hamster
Tissu	Ovary
Maladie	Chinese hamster ovary, non-neoplastic; genetically engineered for CXCR4 surface expression (medium-high expression level)

Organisme	Chinese hamster
Tissu	Ovary
Maladie	Chinese hamster ovary, non-neoplastic; genetically engineered for CXCR7 (ACKR3) surface expression (medium-high expression level)

Organisme	Chinese hamster
Tissu	Ovary
Maladie	Chinese hamster ovary, non-neoplastic; genetically engineered for CTLA-4 surface expression

Organisme	Chinese hamster
Tissu	Ovary
Maladie	Chinese hamster ovary, non-neoplastic; genetically engineered for FOLR1 (Folate Receptor alpha) surface expression