BT-483 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

593,40 CAD$*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

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cryovial

Numéro de produit : 305247

Fiche signalétique

Fiche de produit

Certificat d'analyse (CoA)

Description	The BT-483 cell line is derived from a human breast carcinoma. Specifically, these cells were established from a primary tumor of an adult female patient with breast cancer. BT-483 cells are characterized by their epithelial morphology and are adherent in culture, forming monolayers. This cell line is estrogen receptor-positive (ER+), making it particularly valuable for studying hormone-responsive breast cancers and the role of estrogen in breast cancer progression Researchers use BT-483 cells to investigate various aspects of breast cancer biology, including hormone receptor signaling, gene expression profiles, and the cellular responses to different hormonal therapies. These cells are essential for testing the efficacy of anti-estrogen drugs, such as tamoxifen and aromatase inhibitors, which are commonly used in the treatment of ER+ breast cancers. Additionally, BT-483 cells provide a useful model for studying the mechanisms of drug resistance, tumor growth, and metastasis in hormone-responsive breast cancer. The BT-483 cell line also serves as a tool for high-throughput screening of new therapeutic agents and for understanding the molecular basis of breast cancer heterogeneity. By comparing the responses of BT-483 cells to various treatments with other breast cancer cell lines, researchers can identify specific vulnerabilities and potential targets for therapy, ultimately contributing to the development of more effective and personalized treatments for breast cancer patients.
Organisme	Human
Tissu	Breast
Maladie	Invasive breast carcinoma of no special type
Synonymes	BT483

Âge	23 years
Genre	Female
Origine ethnique	European
Morphologie	Epithelial
Type de cellule	Epithelial cell
Propriétés de croissance	Adherent

Référence	BT-483 (Cytion catalog number 305247)
Niveau de biosécurité	1
NCBI_Numéro d'identification fiscale	9606
Cellosaurus - Numéro d'enregistrement	CVCL_2319

Tumorigène	Yes, in Amsterdam/IMR rats; Yes, in nude mice; Yes, in soft agar
Profil mutationnel	Mutation: PIK3CA, p.Glu542Lys (c.1624G>A); Mutation: TP53 p.Met246Ile (c.738G>A)

Milieu de culture	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Suppléments	Supplement the medium with 10% FBS
Réactif de dissociation	TrypLE Express
Repiquage	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Densité de semis	1 to 3 x 10⁴ cells/cm²
Renouvellement des fluides	2 to 3 times per week
Milieu de congélation	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Décongélation et culture des cellules	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Atmosphère d'incubation	37°C, 5% CO₂, humidified atmosphere.
Conditions d'expédition	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Conditions d'entreposage	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Stérilité	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Numéro de lot	Type de certificat	Date	Numéro de catalogue
305247-070526	Certificat d'analyse	24. Jun. 2026	305247

BT-483 Cells

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Certificat d'analyse (CoA)