Y-79 Cells
General information
Description | The Y79 line was isolated by T.W. Reid and associates in January 1971 by explant culture of a primary tumor from the right eye obtained immediately after enucleation. The donor had a strong maternal family history of retinoblastoma. Ultrastructural features including nuclear membrane infoldings, triple membrane structures, microtubules, large coated vesicles, centrioles, basal bodies and annulate lamellae were reportedly similar to those of the original tumor. |
---|---|
Organism | Human |
Tissue | Retina |
Disease | Retinoblastoma |
Synonyms | Y79, GM01232, GM01232E |
Characteristics
Age | 2,5 years |
---|---|
Gender | Female |
Ethnicity | Caucasian |
Morphology | Multicellular clusters |
Growth properties | Clusters in Suspension |
Identifiers / Biosafety / Citation
Citation | Y-79 (Cytion catalog number 300382) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Isoenzymes | PGM1, 1, G6PD, B, ES-D, 1, AK-1, 1, GLO-1, 2, Phenotype Frequency Product: 0.1373 |
---|---|
Reverse transcriptase | Negative |
Karyotype | Hypertriploid, with abnormalities including dicentrics, breaks, pulverizations and minutes |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 1x10^5 cells/ml |
Fluid renewal | Every 3 days |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
CSF1PO: 11,12
D13S317: 11,12
D16S539: 13,14
D5S818: 11,12
D7S820: 8,9
TH01: 6,9.3
TPOX: 8
vWA: 15,18
D3S1358: 15,16
D21S11: 30,32
D18S51: 13,16
Penta E: 13,18
Penta D: 12
D8S1179: 13,16
FGA: 22
|
HLA alleles |
A*: 02:01:01
B*: 01.01.1900 16:01, 02.01.1900 03:01
C*: 03:04:01, 12:03:01
DRB1*: 01:01:01, 13:02:01
DQA1*: 01:01:01, 01:02:01
DQB1*: 05:01:01, 06:04:01
DPB1*: 03:01, 04:01
E: 01:01, 01:03
|