WT51 Cells
General information
Description | EBV-transformed B-lymphoblastoid cell line, derived from a male person, age unspecified. Homozygous cell line for HLA A:9, ,B:14, DR:4, and DP:2. Consanguineous parents. WT51 was part of the 10th International Histocompatibility Workshop (10IHW) cell line panel. Submitted by Dr.M.Trucco, HLA-Laboratory, Pittsburgh University Cancer Institute, USA. |
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Organism | Human |
Tissue | Peripheral blood |
Applications | Functional analysis and genotyping of HLA Class II molecules. Analysis of B cell surface antigens, testing of cytotoxic drugs, mutational analysis, analysis of apoptotic mechanisms |
Synonyms | WT-51, WT 51, GM03103, GM3103, GM03103A |
Characteristics
Age | Unspecified |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Round cells |
Cell type | B lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | WT51 (Cytion catalog number 302141) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | CD19+ |
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Viruses | Free of human pathogenic viruses SV40, JC/BK, HBV, HCV, and HIV. Contains EBV. |
Karyotype | 46, x,Y |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. |
Split ratio | Inoculate the fresh medium with 5x10^5 cells/ml |
Fluid renewal | 1 to 2 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 10
D13S317: 8,12
D16S539: 11,12
D5S818: 11,13
D7S820: 8,11
TH01: 8,9.3
TPOX: 8,11
vWA: 17,19
D3S1358: 15
D21S11: 30.2,32.2
D18S51: 12,14
Penta E: 7,13
Penta D: 13
D8S1179: 11,12
FGA: 24,25
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HLA alleles |
A*: 23:01:01:01
B*: 14:01:01
C*: 08:02:01:02
DRB1*: 04:01:01
DRB4*: 01:01
DQA1*: 03:01
DQB1*: 03:02:01
DPA1*: 01:03
DPB1*: 02:01:02, 02:01:19
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