WEHI-3B Cells
General information
Description | The growth of WEHI-3 is inhibited by 4 ng/ml LPS and blocked by higher concentrations. Dextran sulfate at 30 to 40 microgram/ml also inhibits growth. Latex beads are phagocytized but are not toxic. Zymosan and BCG are phagocytized and block growth. The cells exhibit only weak effector activity in antibody dependent cell mediated cytotoxicity. |
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Organism | Mouse |
Tissue | Peripheral blood |
Disease | Leukemia |
Synonyms | WEHI-3b, Wehi-3B, WEHI 3B, WEHI3B |
Characteristics
Cell type | Myelomonocyte |
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Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | WEHI-3B (Cytion catalog number 400376) |
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Biosafety level | 2 |
Expression / Mutation
Receptors expressed | Immunoglobulin (Fc), complement (C3) |
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Viruses | Ectromelia virus (mousepox) negative |
Products | Lysozyme, granulocyte colony stimulating activity (G-CSA), interleukin-3 (interleukin 3, IL-3) |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10^5 cells/ml and maintain between 1 x 10^5 and 1x10^6 cells/ml. Adherent cells can be recovered by scraping. |
Seeding density | 1 x 10^5 cells/ml |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | After thawing, allow the cells to recover from the freezing process for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
M_18-3: 17,20
M_4-2: 21.3
M_6-7: 12
M_3-2: 14
M_19-2: 13
M_7-1: 25.2,26.2
M_1-1: 15,16
M_8-1: 13
M_2-1: 16
M_15-3: 22.3
M_6-4: 18
M_11-2: 18,19
M_1-2: 17
M_17-2: 18
M_12-1: 16,17
M_5-5: 14,17
M_X-1: 26
M_13-1: 15,2
Human D4/D8: -
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