UWO37 Cells
General information
Description | The UWO37 (HPV16) cell line originates from the tumor cells of a male patient with oral tongue cancer and is characterized by its expression of HPV16. This particular cell line serves as a critical resource for researchers aiming to understand the intricate relationship between HPV, specifically HPV16, and the development of head and neck squamous cell carcinoma. Utilizing the UWO37 cell line, scientists can explore the molecular dynamics, pathogenic pathways, and potential therapeutic targets associated with HPV16's role in cancer, potentially leading to breakthroughs in treatment and early detection strategies for such malignancies. |
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Organism | Human |
Tissue | Oral cavity; tonsil |
Disease | Squamous cell carcinoma of the oropharynx |
Applications | Generating Cisplatin Resistant HPV-positive HNSCC cell lines to study cisplatin resistance in HPV-positive cells |
Synonyms | University of Western Ontario 37 |
Characteristics
Age | 64 years |
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Gender | Male |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | UWO37 (Cytion catalog number 300257) |
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Biosafety level | 2 |
Expression / Mutation
Viruses | Transformant: Human papillomavirus type 16 (HPV16); weak expression of HPV16 E7 |
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Handling
Culture Medium | DMEM:Ham's F12, w: 3.1 g/L Glucose, w: 1.6 mM L-Glutamine, w: 15 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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