UM-SCC-129 Cells
General information
| Description | UM-SCC-129 is a human head and neck squamous cell carcinoma (HNSCC) cell line established from a patient tumor at the University of Michigan Head and Neck Oncology Laboratory. As part of the UM-SCC cell line panel — one of the most extensively characterized and widely distributed HNSCC cell line collections in the world — UM-SCC-129 provides a defined in vitro model for head and neck squamous cell carcinoma research. The UM-SCC series was developed over 40+ years from over 100 patient donors at the University of Michigan and is distinguished by well-documented STR profiles, TP53 mutation status, and radiation sensitivity data across the collection. UM-SCC-129 is applicable in HNSCC biology research, radiation sensitivity studies, chemotherapy sensitivity testing (cisplatin, 5-FU, cetuximab), EGFR pathway analysis, TP53 functional studies, and evaluation of novel targeted agents. The UM-SCC panel culture is well-established in DMEM with 10% FBS and 1% NEAA, matching standard UM-SCC culture conditions across the collection. As with all UM-SCC lines, STR authentication and TP53 mutation status confirmation are recommended prior to experiments. |
|---|---|
| Organism | Human |
| Tissue | Head and neck |
| Disease | Head and neck squamous cell carcinoma (HNSCC) |
| Metastatic site | Primary tumor site (head and neck) |
| Applications | HNSCC research; radiation sensitivity; cisplatin/5-FU/cetuximab sensitivity; EGFR pathway analysis; TP53 functional studies; targeted therapy evaluation |
Characteristics
| Age | Age unspecified |
|---|---|
| Gender | Sex unspecified |
| Ethnicity | Ethnicity unspecified |
| Morphology | Epithelial-like |
| Cell type | Epithelial cells |
| Growth properties | Adherent |
Regulatory Data
| Citation | UM-SCC-129 (Cytion catalog number 305727) |
|---|---|
| Biosafety level | 1 |
| NCBI_TaxID | 9606 |
| CellosaurusAccession | Not assigned |
| GMO Status | No genetic modification; wildtype HNSCC cell line |
Biomolecular Data
Handling
| Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
|---|---|
| Supplements | Supplement the medium with 10% FBS and 1% NEAA |
| Dissociation Reagent | Accutase |
| Doubling time | approx. 24 to 48 hours |
| Subculturing | Remove medium, wash with PBS without calcium and magnesium, cover with Accutase, incubate 8–10 min at RT, resuspend in medium, centrifuge 300×g 3 min, discard supernatant, reseed in fresh medium. |
| Split ratio | 1 to 5 |
| Seeding density | 1 to 3 × 104 cells/cm2 |
| Fluid renewal | Every 2 to 3 days |
| Freeze medium | As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability. |
| Thawing and Culturing Cells |
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| Incubation Atmosphere | 37°C, 5% CO2, humidified atmosphere. |
| Shipping Conditions | Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage. |
| Storage Conditions | For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen. |