U-343 MG Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$800.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300365

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The U-343 MG cell line is derived from a human glioblastoma, a type of aggressive brain tumor. Originally isolated from a 54-year-old Caucasian male, this cell line has been widely used in neurological research, particularly in studies concerning the pathology and therapeutic treatment strategies for glioblastoma. The U-343 MG cell line is notable for its astrocytic properties, resembling those of astrocytes in the brain, which makes it particularly useful for studying tumor behavior and neurobiology in a controlled in vitro environment. Genetically, the U-343 MG cells are characterized by various mutations typical of glioblastoma, including alterations in the TP53 gene and the EGFR gene. These mutations not only offer insights into the molecular underpinnings of glioblastoma malignancy but also serve as potential targets for therapeutic intervention. The cell line is also used to assess the cytotoxicity of drugs and to study the mechanisms of resistance that glioblastoma cells can develop. This makes U-343 MG a valuable model for evaluating the efficacy of new chemotherapeutic agents and for exploring novel treatment paradigms, such as targeted therapy and immunotherapy.
Organism	Human
Tissue	Brain
Disease	Glioblastoma
Synonyms	U-343MG, U-343-MG, U343MG, U-343, U343, 343 MG, 343MG

Age	54 years
Gender	Male
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth properties	Adherent

Citation	U-343 MG (Cytion catalog number 300365)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_S471

Receptors expressed	GFAP: 95% of the cells tested positive.
Tumorigenic	Yes, in nude mice

Culture Medium	EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a)
Supplements	Supplement the medium with 10% FBS and 1% NEAA
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Seeding density	2 x 10⁴ cells/cm²
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use 50% basal medium + 40% FBS + 10% DMSO, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
300365-240625	Certificate of Analysis	18. Aug. 2025	300365
300365-721	Certificate of Analysis	23. May. 2025	300365

U-343 MG Cells

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)