U-138 MG Cells
General information
Description | This is one of a number of cell lines derived from malignant gliomas, e.g. U-87-MG, U-118-MG and U-373-MG isolated by J. Ponten and associates from 1966 to 1969. It differs from U-87-MG in morphology and it has a slower proliferation rate. U-138-MG shows strong similarity to U-118-MG, sharing at least six derivative marker chromosomes. |
---|---|
Organism | Human |
Tissue | Brain |
Disease | Astrocytoma |
Synonyms | U-138MG, U-138-MG, U138-MG, U 138 MG, U138MG, U138, 138 MG, 138MG |
Characteristics
Age | 47 years |
---|---|
Gender | Male |
Ethnicity | Caucasian |
Morphology | Polygonal |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | U-138 MG (Cytion catalog number 300363) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Antigen expression | Blood Type A, Rh+ |
---|---|
Isoenzymes | Me-2, 1, PGM1, 1, PGM3, 1, ES-D, 1, AK-1, 1, GLO-1, 1-2, G6PD, B, |
Karyotype | Hyperdiploid to pentaploid with several markers, the stemline chromosome number is near triploid with the 2S component occurring at 9.8%. Five markers [t(11,5), t(8q,4), t(19,?18), M1 and M2] were common to most S metaphases. One chromosome 4 could be found in every S metaphase. Chromosome composition was very uniform among cells. Phenotype Frequency Product: 0.0511 |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
CSF1PO: 12
D13S317: 9,11
D16S539: 12,13
D5S818: 11
D7S820: 9
TH01: 6
TPOX: 8
vWA: 18
D3S1358: 15
D21S11: 27,32.2
D18S51: 13
Penta E: 7
Penta D: 9,13
D8S1179: 14,15
FGA: 18,23
|
HLA alleles |
A*: 24:02:01, 29:02:01
B*: 39:06:02, 44:03:01
C*: 07:02:01, 16:01:01
DRB1*: 07:01:01, 08:01:01G
DQA1*: 02:01:01, 04:01:01
DQB1*: 02:02:01, 04:02:01
DPB1*: 04:02:01, 11:01:01
E: 01:01, 01:03
|