TF-1 Cells
General information
Description | The TF-1 cell line has been established by T. Kitamura in October 1987 from a heparinised bone marrow aspiration sample from an erytholeukemic patient. TF-1 cells proliferate depending on the GM-CSF and IL-3. The morphological and cytochemical features, plus the constitutive expression of globin genes, indicate the commitment of the cells to the erythroid lineage. TPA induces a dramatic differentiation into macrophage-like cells. |
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Organism | Human |
Tissue | Bone marrow |
Disease | Erythroleukemia |
Applications | The TF-1 cell line can be applied in various systems due to their responsiveness to multiple cytokines. They provide a good system to investigate the proliferation and differentiation of myeloid progenitor cells. Sensitive to GM-CSF, IL-3, EPO. |
Synonyms | TF1, MFD-1 |
Characteristics
Age | 35 years |
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Gender | Male |
Ethnicity | Japanese |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | TF-1 (Cytion catalog number 300434) |
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Biosafety level | 1 |
Expression / Mutation
Receptors expressed | TF-1 cells do not express glycophorin A or carbonyl anhydrase I. |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS, for long-term culture: IL-3 |
Subculturing | Initiate cultures with a cell density of 2 x 10^5 cells/ml and maintain them within the range of 1 x 10^5 to 1 x 10^6 cells/ml. For subculturing, transfer the cell suspension to a fresh cell culture flask pre-filled with the correct volume of fresh culture medium. |
Seeding density | > 2 x 10^5 cells/ml |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 13
D13S317: 8,9
D16S539: 9,12
D5S818: 13
D7S820: 12
TH01: 7,9
TPOX: 8
vWA: 15,17
D3S1358: 15
D21S11: 30
D18S51: 13
Penta E: 5,17
Penta D: 10,13
D8S1179: 11,15
FGA: 18,19
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HLA alleles |
A*: 02:01:01, 33:03:01
B*: 44:03:01, 51:01:01
C*: 01:02:01, 14:03:01
DRB1*: 09:01:02G, 13:02:01
DQA1*: 01:02:01, 03:02:01
DQB1*: 03:03:02, 06:04:01
DPB1*: 02:01:02, 04:01:01
E: 01:01:01, 01:03:01
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