T84 Cells
General information
Description | This line exhibits tight junctions, and desmosomes between adjacent cells. The cells should be maintained at high density (at least 1/4 confluency). |
---|---|
Organism | Human |
Tissue | Colon |
Disease | Carcinoma |
Metastatic site | Lung |
Synonyms | T-84, T 84 |
Characteristics
Age | 72 years |
---|---|
Gender | Male |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | T84 (Cytion catalog number 300354) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Receptors expressed | Peptide hormone, neurotransmitter |
---|---|
Antigen expression | Keratin + (Immunoperoxidase staining) |
Isoenzymes | G6PD, B, PGM1, 1, PGM3, 1, ES-D, 1, Me-2, 1-2, AK-1, 1, GLO-1, 1-2 |
Tumorigenic | Yes, in nude mice |
Products | Carcinoembryonic antigen (CEA), 600 ng/ml per 10 exp6 cells per 10 days, keratin |
Mutational profile | T84 cells carry a heterozygous Kras mutation in codon13: GGC(Wt Gly) >GAC(Asp) |
Karyotype | The stemline modal chromosome number is 56, occurring at 28% with polyploidy at 12.4%. Eighteen markers are common to most metaphases examined. Normal x and chromosome 13 were absent, chromosomes 2, 4 and 22 were single-copied, and chromosome 12 was 4-copied.No Y chromosome was detected by Q band observation. DM occurred in nearly 50% of the cells. |
Handling
Culture Medium | Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Fluid renewal | 2 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
CSF1PO: 10
D13S317: 9
D16S539: 10,11
D5S818: 12
D7S820: 8,10
TH01: 6,9
TPOX: 8
vWA: 17,18
D3S1358: 19
D21S11: 31
D18S51: 17
Penta E: 14
Penta D: 9
D8S1179: 15
FGA: 24
|
HLA alleles |
A*: 02:01:01, 24:02:01
B*: 18:01:01, 35:01:01
C*: 04:01:01, 07:01:01
DRB1*: 01:01:01, 09:01:02
DQA1*: 01:01:01, 03:02:01
DQB1*: 03:03:02, 05:01:01
DPB1*: 02:01:02, 04:01:01
E: 01:03:01, 01:03:02
|