SW527 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$550.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 300640

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The SW527 cell line is a human breast carcinoma cell line derived from a Caucasian adult patient. It was established in the early 1970s and has been included in foundational studies characterizing tumorigenicity in immunodeficient models. In one such study, SW527 successfully formed tumors in nude mice following subcutaneous inoculation of 6 × 10⁶ cells, supporting its malignant origin. Histopathological analysis of the resulting tumors showed features consistent with the original human carcinoma, confirming its relevance as a breast cancer model. SW527 has been authenticated as a tumor-derived line of G6PD type B, a classification that helps exclude contamination with HeLa cells, which is a critical concern in historical cell line collections. Despite this, comprehensive molecular or immunological profiling of SW527 appears limited in recent large-scale datasets. Overall, SW527 remains a validated breast carcinoma model, primarily supported by in vivo tumorigenicity data. Additional molecular profiling would be beneficial to broaden its utility in mechanistic or drug discovery research.
Organism	Human
Tissue	Breast; Mammary gland
Disease	Breast adenocarcinoma
Synonyms	SW-527, SW 527

Age	70 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial
Cell type	Epithelial
Growth properties	Adherent

Citation	SW527 (Cytion catalog number 300640)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_3799

Mutational profile	Mutation: p.Gln1338Ter, Homozygous; Mutation: p.Gly12Val, Homozygous; Mutation: p.Arg273His, Heterozygous; Mutation: p.Pro309Ser, Heterozygous

Culture Medium	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Freeze medium	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Lot Number	Certificate Type	Date	Catalog Number
300640-120925	Certificate of Analysis	05. Dec. 2025	300640

SW527 Cells

General information

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Regulatory Data

Biomolecular Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)