SVI Cells
$800.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Description | The SVI cell line has been cloned from the outgrowth of glomeruli which were isolated from H-2kb-tsA58 transgenic mice. The mice carry a temperature-sensitive variant of the SV40 large T antigen under control of the IFN-g-inducible H-2kb promoter. Cells proliferate at 33 degree Celsius, and they differentiate at 37 degree Celsius. At present, the cells have been cultured successfully for more than 40 passages without noting phenotypic changes. SVI are very similar to E11 in terms of morphology and the expression of several markers. For example, podocin and WT1 are expressed to a lesser extent as compared to E11. Differentiation: Start the differentiation process by placing the non-confluent flask(s) into an incubator at 38 degree Celsius / 5% CO2 for a minimum of 14 days to complete the differentiation. Addition of interferon-gamma (INF-gamma) is not necessary. |
---|---|
Organism | Mouse |
Tissue | Kidney |
Characteristics
Age | Adult |
---|---|
Gender | Unspecified |
Cell type | Podocyte |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SVI (Cytion catalog number 400495) |
---|---|
Biosafety level | 1 |
Depositor | Dr. N. Endlich |
Expression / Mutation
Protein expression | WT1, Lmx1b, nephrin, NEPHI, FAT, P-cadherin, CD2AP, ZO-I, podocalyxin, podoplanin, synpo, podocin, TRPC6 and GAPDH. |
---|
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:5 is recommended Under differentiation conditions, ie incubation of non to confluent cultures at 38 degree Celsius, cell proliferation ceases within the first two weeks and stops after about four weeks |
Seeding density | Inocculate T75 cell culture flasks with 1x 10^4 cells/cm^2 (about 60.000 cells/ml, 12ml medium in one T75) for the proliferation process. Keep the cells at 33 degree Celsius / 5% CO2, until the flask is about 75% confluent. |
Fluid renewal | 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
|