SV-80 Cells
General information
Description | This SV40-transformed line was originally generated using cells which were derived from a skin biopsy of an adult female (strain A) by Todaro et al. in 1963, not from lung tissue of a five month old male fetus (strain C). After infection, the morphology of the growing colonies changed in that fibroblastic and epitheloid colony types were produced. The designation of SV-80 being of lung origin, and then retained, most probably was invalid. However, this cell line will characterized further in terms of p53 antigen and the presence of large T antigen. |
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Organism | Human |
Tissue | Skin |
Synonyms | SV-80, SV 80, SV-A clone 80, SV clone 80, Simian virus 80 |
Characteristics
Age | Adult |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Cell type | Fibroblast |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SV-80 (Cytion catalog number 300345) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | SMRV: Negative, as confirmed by Real-Time PCR |
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Karyotype | Modal number = 76, range = 52 to 87 |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 20 to 24 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 is recommended |
Fluid renewal | 1 to 2 times per week |
Freezing recovery | Fast |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 12
D13S317: 12
D16S539: 9,13
D5S818: 12
D7S820: 10
TH01: 9
TPOX: 10,11
vWA: 16
D3S1358: 16
D21S11: 28,30
D18S51: 15,20
Penta E: 11,12
Penta D: 9
D8S1179: 11,15
FGA: 21,27
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HLA alleles |
A*: 02:01:01, 03:01:01
B*: 15:10:01, 45:01:01
C*: 03:04:02, 16:01:01
DRB1*: 10:01:01, 13:02:01
DQA1*: 01:02:01, 01:05:01
DQB1*: 05:01:01
DPB1*: 01:01:01, 04:02:01G
E: 01:01, 01:03
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