SKW-3 Cells
General information
Description | The SKW-3 cell line was thought to originate from the blood of a 61-year-old male patient with chronic lymphocytic leukemia (CLL). However, STR authentication has revealed that this cell line is contaminated and therefore is considered a derivate of KE-37 cells. |
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Organism | Human |
Tissue | Hematopoietic |
Disease | T cell leukemia (CLL) |
Synonyms | SKW3 |
Characteristics
Age | 27 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Round cells |
Cell type | T Lymphocyte |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | SKW-3 (Cytion catalog number 300343) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | CD2+, CD3-, CD4+, CD8, Thy-1-like antigen |
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Products | LECT2 (chemotactic protein) |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Doubling time | 30 hours |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Freezing recovery | 1 x 10^5/ml |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 10,12
D13S317: 8,12
D16S539: 11,12
D5S818: 12,13
D7S820: 8,12
TH01: 6,9.3
TPOX: 8
vWA: 17,18
D3S1358: 15,18
D21S11: 28,29,39
D18S51: 13,18
Penta E: 5,14
Penta D: 11,15
D8S1179: 11,14
FGA: 24,25
D1S1656: 15.3,16
D6S1043: 18,21
D2S1338: 19,25
D12S391: 19,22
D19S433: 13,15
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HLA alleles |
A*: 11:01:01, 30:01:01
B*: 35:01:01, 44:02:01
C*: 04:01:01, 05:01:01
DRB1*: 01:03:01, 04:01:01
DQA1*: 01:01:01, 03:03:01
DQB1*: 03:01, 05:01
DPB1*: 04:01:01, 04:02:01
E: 01:01:01
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