SK-BR-3-Luc

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
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USD$800.00*

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cryovial

Product number: 305709

Safety data sheet

Product sheet

Description	SK-BR-3-Luc is a bioluminescent derivative of the human SK-BR-3 breast adenocarcinoma cell line, engineered to stably express a firefly luciferase reporter gene. The parental SK-BR-3 cell line was established from a pleural effusion metastasis of a breast adenocarcinoma in a 43-year-old Caucasian female patient and is characterized by HER2 (ERBB2) gene amplification and overexpression, classifying it as a HER2-positive breast cancer model. SK-BR-3 cells exhibit epithelial-like monolayer growth and are widely used to study HER2-driven breast cancer biology and to evaluate HER2-targeted therapies including trastuzumab, lapatinib, pertuzumab, and antibody-drug conjugates such as trastuzumab-deruxtecan. The stable luciferase integration in SK-BR-3-Luc enables sensitive, quantitative bioluminescence imaging (BLI) of tumor burden in xenograft models in immunocompromised hosts. The emitted luminescent signal correlates with viable tumor cell number, supporting noninvasive longitudinal monitoring of tumor engraftment, growth kinetics, and therapeutic response. SK-BR-3-Luc is particularly valuable for preclinical evaluation of anti-HER2 agents, ADCs, bispecific antibodies, and combination strategies targeting the HER2 signaling axis in breast cancer, as well as for high-throughput drug screening and in vivo pharmacodynamic studies. SK-BR-3-Luc retains the established HER2-amplified molecular phenotype and growth characteristics of the parental SK-BR-3 line. The luciferase reporter substantially enhances experimental throughput and sensitivity for in vivo imaging studies. Researchers should confirm luciferase activity, HER2 amplification status, and growth kinetics under their specific experimental conditions prior to large-scale preclinical use.
Organism	Human
Tissue	Metastatic
Disease	Breast adenocarcinoma
Metastatic site	Pleural effusion

Age	43 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Growth properties	Monolayer, adherent

Citation	SK-BR-3-Luc (Cytion catalog number 305709)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_4Y11
GMO Status	GMO-S1: This cell line contains a stably integrated firefly luciferase reporter cassette (Luc2, codon-optimized) introduced via replication-incompetent lentiviral transduction. The resulting polyclonal cell population was maintained under puromycin selection (1–5 µg/mL). S1 containment is required. This classification applies only within Germany and may differ elsewhere.

Antigen expression	Blood Type A, Rh+, HLA A11, Bw22(+/-), B40, B18, Luc2 (firefly, codon-optimized)
Isoenzymes	PGM3, 1, PGM1, 1-2, ES-D, 1, AK-1, 1-2, GLO-1, 2, G6PD, B, Phenotype Frequency Product: 0.0044
Tumorigenic	Yes, in nude mice, forms poorly differentiated adenocarcinoma
Mutational profile	Mutation: p.Arg175His, Homozygous

Culture Medium	McCoys 5A medium (modified), w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3 (Cytion 820200a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase
Subculturing	Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium.
Split ratio	1 to 3
Seeding density	2-4*10^4 cells/cm²
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium + 10% DMSO for adequate post-thaw viability.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 200 x g for 5 minutes, carefully discard the supernatant containing freezing medium. Follow the procedure described under Post-Thaw Recovery
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Properties "SK-BR-3-Luc"

Metastatic site:	Pleural effusion

SK-BR-3-Luc

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Quality Control & Molecular Analysis