SCLC-22H Cells
General information
Organism | Human |
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Tissue | Lung |
Disease | Small cell carcinoma |
Metastatic site | Pericardial effusion |
Synonyms | SCLC22H |
Characteristics
Age | 46 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Floating cell aggregates, few single cells |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | SCLC-22H (Cytion catalog number 300445) |
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Biosafety level | 1 |
Depositor | K?hler |
Expression / Mutation
Tumorigenic | Yes, in nude mice |
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Reverse transcriptase | Negative |
Karyotype | Modal number 43 |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Split ratio | A ratio of 1:2 to 1:6 is recommended |
Seeding density | 1 x 10^5 cells/ml |
Fluid renewal | 1 to 2 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 10
D13S317: 12
D16S539: 12
D5S818: 11,12
D7S820: 11
TH01: 9.3
TPOX: 8,9
vWA: 17,18
D3S1358: 15
D21S11: 29,31.2
D18S51: 14,15
Penta E: 12,13
Penta D: 9
D8S1179: 12,13
FGA: 22
|
HLA alleles |
A*: 01:01:01, 32:01:01
B*: 27:05:02, 51:01:01
C*: 02:02:02
DRB1*: 04:01:01, 09:01:02G
DQA1*: 03:01:01, 03:02:01
DQB1*: 03:02:01, 03:03:02
DPB1*: 02:01:02, 04:01:01
E: 01:01:01
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