SCL II Cells
$800.00*
Prices excl. VAT plus shipping costs This cell line is licensed to CLS by a university or institute. The sale of this item requires the conclusion of a Material Transfer Agreement (MTA). Please get in touch with us for further information.
General information
Description | The human cell line SCL-II was established from an undifferentiated squamous cell carcinoma of a patient by Boukamp et al. in 1983. |
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Organism | Human |
Tissue | Skin, Face |
Disease | Squamous cell carcinoma |
Synonyms | SCL-II, SCL-2, SCL2 |
Characteristics
Age | 91 years |
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Gender | Male |
Ethnicity | Caucasian |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | SCL II (Cytion catalog number 300497) |
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Biosafety level | 1 |
Depositor | DKFZ, Heidelberg |
Expression / Mutation
Protein expression | P53 (+) |
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Tumorigenic | Formation of highly differentiated, locally invasive squamous cell carcinoma in Balb/c-nu/nu mice. |
Karyotype | Aneuploid (hypodiploid, few metaphases hypotetraploid) |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | TrypLE Express (Life Technologies) |
Doubling time | 40 to 50 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:5 to 1:10 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 2 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 12
D13S317: 8,12
D16S539: 10,11
D5S818: 9
D7S820: 8,12
TH01: 8
TPOX: 8,11
vWA: 15,17
D3S1358: 14
D21S11: 29
D18S51: 17
Penta E: 13
Penta D: 9,13
D8S1179: 12,13
FGA: 26
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HLA alleles |
A*: 68:02:01
B*: 07:02:01, 07:02:01
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