RenCa Cells
General information
Description | The RenCa cell line has been established from the murine transplantable renal adenocarcinoma of spontaneous origin. |
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Organism | Mouse |
Tissue | Kidney |
Disease | Carcinoma |
Synonyms | Renca, RENCA, Renal Carcinoma |
Characteristics
Age | 6 weeks |
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Gender | Male |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | RenCa (Cytion catalog number 400321) |
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Biosafety level | 1 |
Expression / Mutation
Tumorigenic | Yes, in syngeneic mice |
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Virus susceptibility | MAP testing negative (Sendai, Ektromelie, Polyoma, K-Virus, Kilham, LCM, M.pulmonis, MVM, Theiler`s GD VII, toolan`s H-1, MHV, RCV/SDA, M-Adenovirus) |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 47 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:8 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | Fast. Viability 93%. Allow cells to recover from the freezing process for 24 to 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
M_18-3: 18,20,21,22
M_4-2: 21.3
M_6-7: 12
M_3-2: 14,15
M_19-2: 13,14
M_7-1: 23.2,25.2
M_1-1: 15,16,17,18
M_8-1: 13
M_2-1: 15,16,17
M_15-3: 22.3,23.3
M_6-4: 18,19
M_11-2: 17,18
M_1-2: 16,18,19
M_17-2: 15,17
M_12-1: 16,17
M_5-5: 14,15,16
M_X-1: 25
M_13-1: 16.2
Human D4/D8: -
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