RT-4 Cells
General information
Description | This cell line was established in 1970 by Rigby et al. |
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Organism | Human |
Tissue | Bladder |
Disease | Transitional cell papilloma |
Synonyms | RT4, RT4P |
Characteristics
Age | 63 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | RT-4 (Cytion catalog number 300326) |
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Biosafety level | 1 |
Expression / Mutation
Protein expression | p53 positive |
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Antigen expression | HLA A25(10), A3, B12, Cw3, Blood Type O |
Isoenzymes | Me-2, 1, PGM1, 1-2, PGM3, 1-2, ES-D, 1-2, AK-1, 1, GLO-1, 1-2, G6PD, B, Phenotype Frequency Product: 0.0050 |
Tumorigenic | Yes, in cheek pouch of steroid treated hamsters |
Karyotype | (P174) hyperdiploid and hypotetraploid to hypertetraploid with abnormalities including dicentrics, breaks, translocations and minutes |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 10,12
D13S317: 8
D16S539: 9
D5S818: 11,12
D7S820: 9
TH01: 9,9.3
TPOX: 8,11
vWA: 14,17
D3S1358: 15
D21S11: 30,32.2
D18S51: 15,17
Penta E: 7,10
Penta D: 12
D8S1179: 13,15
FGA: 22,24
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HLA alleles |
A*: 02:01:01, 03:01:01
B*: 44:02:01
C*: 05:01:01
DRB1*: 04:01:01, 14:54:01
DQA1*: 01:04:01, 03:03:01
DQB1*: 03:01:01, 05:03:01
DPB1*: 04:01:01, 682:01
E: 01:01, 01:03
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