RLE-6TN Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

Cytion GmbH

USD$395.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305350

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	The RLE-6TN cell line is an immortalized rat alveolar type II epithelial cell line derived from adult Fischer 344 rats. RLE-6TN was established through spontaneous immortalization during attempts to introduce the SV40-T antigen gene into primary alveolar type II epithelial cells. Unlike its counterpart RLE-6T, which was positively transfected with the SV40-T antigen, RLE-6TN cells do not express the T-antigen gene. Despite this, RLE-6TN cells retain critical morphological and functional features characteristic of alveolar type II cells, including cytokeratin expression and the presence of lipid-containing lamellar inclusion bodies. RLE-6TN cells have been widely used as an in vitro model for investigating lung epithelial cell biology, alveolar function, and responses to various physiological and pathological stimuli. They are particularly relevant for studying Na-K-ATPase regulation and activity in alveolar epithelial cells. Na-K-ATPase is essential for maintaining cellular ion gradients and trans-epithelial ion transport, processes critical for alveolar fluid clearance in the lungs. In studies, thyroid hormone (T3) has been shown to stimulate Na-K-ATPase activity in RLE-6TN cells by enhancing its translocation to the plasma membrane rather than increasing its transcription, highlighting a novel, rapid regulatory mechanism. RLE-6TN cells demonstrate stable growth, with near-diploid karyotype stability, and are not tumorigenic in nude mice. They are negative for alkaline phosphatase activity but stain positive for cytokeratins 8, 18, and 19, confirming their epithelial origin. RLE-6TN cells can be maintained long-term in culture and serve as a reliable platform for mechanistic studies on alveolar epithelial repair, surfactant metabolism, and cellular responses to lung injury, toxins, and therapeutic agents.
Organism	Rat
Tissue	Lung
Synonyms	Rat Lung Epithelial-6-T-antigen Negative

Age	56 days
Gender	Male
Morphology	Epithelial
Growth properties	Adherent

Citation	RLE-6TN (Cytion catalog number 305350)
Biosafety level	1
NCBI_TaxID	10116
CellosaurusAccession	CVCL_4693

Antigen expression	Cytokeratin 8; cytokeratin 19
Tumorigenic	No, No not tumorgenic in nude mice
Viruses	SV40
Karyotype	Cells are reported to remain near diploid and karyotypically stable from passage 19-70 with 50% or more of the cells containing 42 chromosomes. At passage 37, there was a translocation between chromosome 1 and 15 which results in trisomy of the q arm of chromosome 1.

Culture Medium	DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a)
Supplements	Supplement the medium with 5% FBS
Dissociation Reagent	Accutase
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305350-150125	Certificate of Analysis	23. May. 2025	305350

RLE-6TN Cells

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)