RJ2.2.5 Cells
General information
Description | Established from an 11-year-old boy with Burkitt lymphoma. This cell line is a variant of Burkitt cell line Raji. This cell line is deficient in expression of HLA Class II antigen. RJ2.2.5 is deleted at least in 1 allele of the MHC Class II transactivator (CIITA), the other allele of CIITA is also affected but not completely deleted. RJ2.2.5 is EBNA positive of Epstein Barr virus. |
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Organism | Human |
Tissue | Hematopoietic |
Disease | Burkitt lymphoma |
Applications | Analysis of B cell surface antigens, testing of cytotoxic drugs, mutational analysis, analysis of apoptotic mechanisms, HLA-typing |
Synonyms | Rj2.2.5, RJ-2.2.5, RJ 2.2.5, RJ2.25, Raji 2.2.5 |
Characteristics
Age | 11 years |
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Gender | Male |
Ethnicity | African, Nigerian |
Morphology | Round cells |
Cell type | B lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | RJ2.2.5 (Cytion catalog number 300360) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | CD10+, CD19+ |
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Karyotype | 46, hypodiploid |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Seeding density | 3 x 10^5 cells/ml |
Fluid renewal | 2 times per week |
Freezing recovery | Fast (48 hours) |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 10,12
D13S317: 13,13
D16S539: 8,11
D5S818: 10,10
D7S820: 10,10
TH01: 6,7
TPOX: 8,13
vWA: 16,19
D3S1358: 15,16
D21S11: 28,31
D18S51: 17
Penta E: 5,13
Penta D: 3.2,9
D8S1179: 14,15
FGA: 19,27
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HLA alleles |
A*: 03:01:01
B*: 15:10:01
C*: 03:04:02, 04:01:01
DRB1*: 03:01:01, 10:01:01
DQA1*: 01:05:01, 05:01:01
DQB1*: 02:01:01, 05:01:01
DPB1*: 01:01:01
E: 01:01:01
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