REH Cells
General information
Description | According to our STR-Analysis and comparison with various databanks, the DNA results are ambiguous. |
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Organism | Human |
Tissue | Peripheral blood |
Disease | Acute lymphoblastic leukemia (ALL) |
Synonyms | Reh |
Characteristics
Age | 15 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Round cells |
Cell type | Lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | REH (Cytion catalog number 300320) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | CD3 A (17%) B (17%) C (20%), CD4 (15%), CD10 (55%), CD20+, HLA-DR+, CALLA+ |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 2 x 10^5 cells/ml and keep the cell concentration within the range of 1 x 10^5 to 1 x 10^6 cells/ml for optimal growth. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 12,13
D13S317: 11,12,13
D16S539: 9,10,13
D5S818: 11,12,13
D7S820: 9,11
TH01: 7
TPOX: 8
vWA: 14,15
D3S1358: 18
D21S11: 18
D18S51: 10,13,14
Penta E: 10,11
Penta D: 9,10,12
D8S1179: 13,16
FGA: 22,23,24
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HLA alleles |
A*: 23:01:01, 32:01:01
B*: 35:02:01, 50:01:01
C*: 04:01:01, 06:02:01
DRB1*: 11:01:01, 12:01:01
DQA1*: 05:05:01
DQB1*: 03:01:01
DPB1*: 04:01:01G, 04:02:01G
E: 01:01:01, 01:03:05
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