RD-ES Cells
General information
Description | The cell line was initiated by G. Marshall and M. Kirchen from a primary osseous Ewing?s sarcoma of the humerus. Ultrastructurally, the cells exhibit primitive cell junctions, possess glycogen pools and are 20 to 25 microns in diameter. The cells grow as a loosely attached monolayer in small clusters of 5 to 10 cells. The cells form a loose adherent layer when cultured in EMEM. |
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Organism | Human |
Tissue | Bone |
Disease | Ewing's Sarcoma |
Synonyms | RDES, RDES-1 |
Characteristics
Age | 19 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | RD-ES (Cytion catalog number 300410) |
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Biosafety level | 1 |
Expression / Mutation
Antigen expression | Blood type B, Rh+ |
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Isoenzymes | G6PD, B, PGM1, 1-2, PGM3, 1, ES-D, 1, Me-2, 1-2, AK-1, 1, GLO-1, 1-2, Phenotype Frequency Product: 0.0359 |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:8 is recommended |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 11,11
D13S317: 11,12
D16S539: 9,11
D5S818: 11,11
D7S820: 10,10
TH01: 7,7
TPOX: 9,11
vWA: 17,17
D3S1358: 15,15
D21S11: 28,28
D18S51: 14,18
Penta E: 11,13
Penta D: 9,12
D8S1179: 13,13
FGA: 21,25
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