RCC-WK Cells
General information
Description | Established from the kidney clear cell carcinoma pT3b, No, Mx/ GII of a 75 years old male in 1999. |
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Organism | Human |
Tissue | Kidney |
Disease | Clear cell renal cell carcinoma, pT3b, No, Mx/ GII |
Synonyms | KTCTL-87, KTCTL87, RCCWK |
Characteristics
Age | 75 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | RCC-WK (Cytion catalog number 300243) |
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Biosafety level | 1 |
Depositor | Prof. S. Pomer |
Expression / Mutation
Protein expression | IL8 |
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Mutational profile | IL8 RS1126647 3-UTR SNP A>T |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:3 to 1:8 is recommended |
Fluid renewal | 1 to 2 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 12,11
D13S317: 12
D16S539: 10,12
D5S818: 11
D7S820: 9,12
TH01: 8,9
TPOX: 9,12
vWA: 14,16
D3S1358: 16
D21S11: 28,31.2
D18S51: 17
Penta E: 5,16
Penta D: 12,15
D8S1179: 11,12
FGA: 21,23
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HLA alleles |
A*: 11:01:01, 30:01:01
B*: 13:02:01, 35:01:01
C*: 04:01:01, 06:02:01
DRB1*: 04:07:01, 07:01:01
DQA1*: 02:01:01, 03:03:01
DQB1*: 02:02:01, 03:01:01
DPB1*: 03:01:01, 04:02:01
E: 01:01:01
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