RCC-PR Cells
General information
Description | Established from kidney carcinoma. |
---|---|
Organism | Human |
Tissue | Kidney |
Disease | Clear cell renal cell carcinoma |
Characteristics
Age | 81 years |
---|---|
Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | RCC-PR (Cytion catalog number 300267) |
---|---|
Biosafety level | 1 |
Depositor | Prof. S. Pomer |
Expression / Mutation
Protein expression | IL8 |
---|---|
Mutational profile | IL8 RS1126647 3-UTR SNP A>T |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
CSF1PO: 11,12
D13S317: 11
D16S539: 12
D5S818: 9
D7S820: 10
TH01: 9
TPOX: 8
vWA: 17
D3S1358: 15,18
D21S11: 29,31.2
D18S51: 12,18
Penta E: 7
Penta D: 11,12
D8S1179: 13,15
FGA: 20,22
|