RCC-MF Cells
General information
Description | Established from the Renal clear cell carcinoma pT2, N1, Mx/ GII-III (lung-metastasis) of a 63 years old male by Pomer et al. in 1997. Cells are G250 positive. |
---|---|
Organism | Human |
Tissue | Kidney |
Disease | Clear cell renal cell carcinoma, pT2, N1, Mx/ GII-III (lung- metastasis |
Synonyms | KTCTL-1M, KTCTL1M, RCCMF |
Characteristics
Age | 63 years |
---|---|
Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | RCC-MF (Cytion catalog number 300245) |
---|---|
Biosafety level | 1 |
Depositor | Prof. S. Pomer |
Expression / Mutation
Surface antigens | Cytokeratine positive 8,18,19, vimentin positive |
---|---|
Receptors expressed | CAIx+ |
Protein expression | p53 positive, G250 positive, IL8 |
Tumorigenic | Yes, in nude mice |
Mutational profile | IL8 RS1126647 3-UTR SNP T>T |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:3 is recommended |
Seeding density | 2 to 3 x 10^4 cells/cm^2 |
Fluid renewal | 1 to 2 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
CSF1PO: 10
D13S317: 11,14
D16S539: 13
D5S818: 11,12
D7S820: 9,10
TH01: 6
TPOX: 8,11
vWA: 18
D3S1358: 17
D21S11: 29,30
D18S51: 13,15
Penta E: 7,12
Penta D: 12,16
D8S1179: 10,14
FGA: 20
|
HLA alleles |
A*: 01:01:01, 02:819
B*: 08:01:01, 15:01:01
C*: 03:03:01, 07:01:01
DRB1*: 03:01:01, 13:01:01
DQA1*: 01:03:01, 05:01:01
DQB1*: 02:01:01, 06:03:01
DPB1*: 04:01:01
E: 01:01:01
|