RCC-JF Cells
General information
Description | Established from the kidney clear cell carcinoma pT2, pM1, No/GII-III of a 65-years-old female, 1999 |
---|---|
Organism | Human |
Tissue | Kidney |
Disease | Clear cell renal cell carcinoma |
Synonyms | KTCTL-140, KTCTL140, RCCJF |
Characteristics
Age | 65 years |
---|---|
Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | RCC-JF (Cytion catalog number 300274) |
---|---|
Biosafety level | 1 |
Depositor | Prof. S. Pomer |
Expression / Mutation
Protein expression | IL8 |
---|---|
Mutational profile | IL8 RS1126647 3-UTR SNP T>T |
Handling
Culture Medium | McCoys 5a, w: 3.0 g/L Glucose, w: stable Glutamine, w: 2.0 mM Sodium pyruvate, w: 2.2 g/L NaHCO3 (Cytion article number 820200a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:3 is recommended |
Fluid renewal | 1 to 2 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
Amelogenin: x,x
CSF1PO: 10,13
D13S317: 11,12
D16S539: 11
D5S818: 11
D7S820: 7,8
TH01: 7.9
TPOX: 8
vWA: 18
D3S1358: 17
D21S11: 28,30.2
D18S51: 13,15
Penta E: 7,13
Penta D: 10,13
D8S1179: 12,14
FGA: 18,25
|
HLA alleles |
A*: 03:01:01
B*: 37:01:01, 51:01:01
C*: 06:02:01, 07:02:01
DRB1*: 11:01:01, 15:01:01G
DQA1*: 01:02:01, 05:05:01
DQB1*: 03:01:01, 06:02:01
DPB1*: 04:01:01, 13:01:01
E: 01:01:01
|