RCC-FG2 Cells
General information
Description | Established from the renal clear cell carcinoma of a 77-years-old-male, pT2a, Nx, M1/ GII. HLA-A2 positive, PAS positive, G250 positive. |
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Organism | Human |
Tissue | Kidney |
Disease | Clear cell renal cell carcinoma, pT2a, Nx, M1/GII |
Synonyms | KTCTL-26A, KTCTL-26a, KTCTL26A, RCCFG2 |
Characteristics
Age | 77 years |
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Gender | Male |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Identifiers / Biosafety / Citation
Citation | RCC-FG2 (Cytion catalog number 300249) |
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Biosafety level | 1 |
Depositor | Prof. S. Pomer |
Expression / Mutation
Surface antigens | Cytokeratine positive 8,18,19, vimentin positive |
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Receptors expressed | CAIx -/+, two peaks in FACS analysis, MAB2188. |
Protein expression | IL8 |
Tumorigenic | In nude mice |
Ploidy status | Aneuploid |
Mutational profile | IL8 RS1126647 3-UTR SNP A>T |
Karyotype | 47,x, -Y,del(2)(p21),del(3)(p14), t(3,13)(p23,q32), +5, +7,der(9)t(5,9)(:q15->q33::p22), +16, -21, -22 (H?gemann, 1994) |
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 24 to 48 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:3 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 |
Fluid renewal | 1 to 2 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
CSF1PO: 11
D13S317: 11,12
D16S539: 11,13
D5S818: 10,12
D7S820: 11,12
TH01: 9
TPOX: 8,11
vWA: 18,19
D3S1358: 16
D21S11: 29,30
D18S51: 15,17
Penta E: 12,18
Penta D: 9,13
D8S1179: 12,15
FGA: 19,23
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HLA alleles |
A*: 03:01:01, 32:01:01
B*: 27:05:02, 35:01:01
C*: 02:02:02, 04:01:01
DRB1*: 01:01:01, 15:01:01G
DQA1*: 01:01:01, 01:02:01
DQB1*: 05:01:01, 06:02:01
DPB1*: 04:01:01
E: 01:01:01, 01:06:01
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