RAW 264.7 Cells
General information about the RAW264.7 cell line
Description | RAW 264.7 cells are a widely used murine macrophage cell line derived from the ascites of a male mouse with a tumor induced by the Abelson murine leukemia virus and are commonly used in immunological and infectious disease research. As an immortalized cell line, RAW264.7 cells are a key model system for studying macrophage biology, including immune responses to pathogens, signal transduction, and gene expression. RAW264.7 cells are particularly valuable for their ability to differentiate into macrophage-like cells. These cells can be polarized into M1 macrophages, associated with inflammatory responses, or M2 macrophages, linked to tissue repair and anti-inflammatory processes. This polarization capacity, along with their ability to perform essential macrophage functions like pinocytosis and phagocytosis, underscores their relevance in studying macrophage biology and the complex interplay between immune responses and pathogens. RAW 264.7 cells are instrumental in studying the immune system's interactions with various factors, including pathogens and bone biology. RAW264.7 cells can be induced to differentiate into osteoclast-like cells under certain conditions, such as exposure to RANKL (Receptor Activator of Nuclear Factor κB Ligand), making them a model for studying certain aspects of osteoclast biology and bone resorption. The RAW264.7 cell line's response to various stimuli, including the induction of pyroptosis, an inflammatory cell death process triggered by factors such as LPS (lipopolysaccharide), is instrumental in dissecting the pathways leading to inflammatory cytokine production. The impact of environmental conditions, such as extracellular glucose levels on cell function and phenotype, offers insights into cellular metabolism and the potential downregulation of inflammatory responses. RAW264.7 cells, with their origins in murine leukemia and their extensive use in immunological research, serve as a crucial tool in advancing our understanding of macrophage biology, immune system-pathogen dynamics, osteoimmunology, and inflammatory responses, highlighting their indispensable role in both basic and applied biomedical research. |
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Organism | Mouse |
Tissue | Ascites |
Disease | Leukemia |
Synonyms | RAW264, RAW2647, RAW264.7, RAW-264.7, Raw 264.7, Raw264.7 |
Properties
Age | Adult |
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Gender | Male |
Cell type | Macrophage |
Growth properties | Adherent |
Documentation
Citation | RAW 264.7 (Cytion catalog number 400319) |
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Biosafety level | 2 |
Genetic profile of the mouse macrophage cell line RAW264.7
Receptors expressed | Immunoglobulin (Fc), complement (C3) |
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Antigen expression | H-2d |
Viruses | The cell line was tested and found positive for Reverse Transcriptase (RT) activity from C-Type retroviruses in cell culture supernatant and cell extract. Ectromelia virus (mousepox) may be secreted. |
Products | Lysozyme |
Cell maintenance techniques
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | RAW264.7 cells exhibit a doubling time ranging from 11 to 30 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Seeding density | 4 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,y
M_18-3: 18
M_4-2: 22.3,23.3
M_6-7: 12
M_3-2: 14
M_19-2: 12,14
M_7-1: 25.2
M_1-1: 15,16
M_8-1: 13
M_2-1: 16
M_15-3: 22.3
M_6-4: 18
M_11-2: 17
M_1-2: 17
M_17-2: 14,16
M_12-1: 16,17
M_5-5: 14
M_X-1: 25
M_13-1: 16.2
Human D4/D8: -
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