RAJI Cells
General information
Description | Raji cells are a line of lymphoblast-like cells established by R.J.V. Pulvertaft in 1963 from Burkitt's lymphoma. These cells are widely used in immunology research due to their high expression of human CD19, which acts as a co-receptor and decreases the threshold for antigen B-cell receptor (BCR) stimulation. Raji cells are non-adherent and grow in suspension as free-floating individuals or doublets. The doubling time of these cells is 23.2 hours, and they are relatively small in diameter with a diameter range of 5-8 ?m. Some characteristics of Raji cells include a lack of differentiation, as they form large aggregations of hundreds of individual cells. These cells are diploid and have a stable karyotype within the male diploid stemline of 46. Additionally, Raji cells are partially resistant to poliovirus and vesicular stomatitis viruses. Human CD19 is highly expressed by Raji cells and has been identified as a clinical target for anti-hCD19-CD3 bis-specific antibodies in non-Hodgkin's B cell lymphoma. BCMA expression has also been identified in the Raji Burkitt lymphoma cell line and primary lymphoma, making it an important area of research for immunologists. |
---|---|
Organism | Human |
Tissue | Maxilia |
Disease | Burkitt lymphoma |
Synonyms | Raji, P1-Raji, GM04671 |
Characteristics
Age | 11 years |
---|---|
Gender | Male |
Ethnicity | African, Nigerian |
Cell type | Lymphoblast |
Growth properties | Suspension |
Identifiers / Biosafety / Citation
Citation | RAJI (Cytion catalog number 300359) |
---|---|
Biosafety level | 1 |
Expression / Mutation
Products | The cells may produce interferon when stimulated by Newcastle disease virus. |
---|
Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
---|---|
Medium supplements | Supplement the medium with 10% FBS |
Subculturing | Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 10^5 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
|
Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
---|---|
STR profile |
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
TH01: 6,7
TPOX: 8,13
vWA: 16,19
D3S1358: 15,16
D21S11: 28,31
D18S51: 17
Penta E: 5,13
Penta D: 3.2,9
D8S1179: 14,15
FGA: 19,27
|
HLA alleles |
A*: 03:01:01
B*: 15:10:01
C*: 03:04:02, 04:01:01
DRB1*: 03:01:01, 10:01:01
DQA1*: 01:05:01, 05:01:01
DQB1*: 02:01:01, 05:01:01
DPB1*: 01:01:01
E: 01:01:01
|