Panc 05.04 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$540.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305394

Safety data sheet

Product sheet

Description	The Panc 05.04 cell line is a pancreatic ductal adenocarcinoma (PDAC) model that serves as a valuable resource for studying pancreatic cancer biology and therapeutic responses. Originating from a human pancreatic carcinoma, Panc 05.04 represents a critical tool in preclinical research due to its relevance to one of the most aggressive malignancies with poor patient outcomes. This cell line displays molecular and genomic characteristics that align with the heterogeneous nature of PDAC, including mutations commonly observed in KRAS, TP53, and other driver oncogenes that are prevalent in pancreatic cancer progression. Panc 05.04 has been extensively profiled as part of large-scale genomic initiatives, such as the Cancer Cell Line Encyclopedia (CCLE), where its gene expression, copy number variations, and drug sensitivity have been characterized. Notably, Panc 05.04 has been assessed for its pharmacological response to various anticancer agents, offering insights into its sensitivity or resistance profiles to chemotherapy and targeted therapies. As a model system, Panc 05.04 facilitates investigations into the molecular mechanisms underlying PDAC progression, chemoresistance, and tumor microenvironment interactions. It also provides a platform for testing emerging targeted therapies and immunotherapeutic strategies aimed at overcoming the refractory nature of pancreatic cancers.
Organism	Human
Tissue	Pancreas
Disease	Adenocarcinoma
Applications	3D cell culture
Synonyms	Panc-05.04, Panc_05_04, Panc05.04, Panc 5.04, Panc5.04, PANC0504, Panc0504, Pa18C, PL-18, PL18

Age	77 years
Gender	Female
Ethnicity	Caucasian
Morphology	Epithelial-like
Cell type	Epithelial cell
Growth properties	Adherent

Citation	Panc 05.04 (Cytion catalog number 305394)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCl_1637

Antigen expression	MHC class I +; MHC class II -; Blood type B; Rh+
Oncogenes	K-ras+
Tumorigenic	Yes; Yes in nude or SCID mice
Mutational profile	Mutation: KRAS, Simple, p.Gly12Asp (c.35G>A), Heterozygous

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 15% FBS
Dissociation Reagent	Trypsin-EDTA
Doubling time	46 hours
Subculturing	For routine adherent cell culture: Aspirate the old culture medium from the adherent cells, and wash them with PBS to remove any remaining medium. After aspirating the PBS, add the appropriate volume of Trypsin/EDTA solution based on the culture vessel size (e.g., 1 ml for a T25 flask, 3 ml for a T75 flask) and incubate at room temperature or 37°C until the cells detach (5-10 minutes). Monitor detachment under a microscope, and gently tap the vessel if necessary to release the cells. Once detached, add complete medium to inactivate the Trypsin/EDTA, gently resuspend the cells, and transfer an aliquot of the cell suspension into a new culture vessel containing fresh medium. Place the vessel in an incubator set to 37°C with 5% CO₂, and change the medium every 2-3 days.
Fluid renewal	1 to 2 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Panc 05.04 Cells

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