PSN-1 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
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USD$375.00*

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cryovial

Product number: 305618

Safety data sheet

Product sheet

Description	The PSN-1 cell line is a human pancreatic adenocarcinoma model established from a primary pancreatic tumor. It is particularly notable for harboring amplifications of both the c-myc oncogene and the c-Ki-ras gene with a point mutation at codon 12. This mutation substitutes arginine for glycine (GGT to CGT), leading to an activated form of c-Ki-ras with transforming potential. PSN-1 retains the same molecular characteristics as the original tumor tissue, including a 50-fold amplification of c-myc and a 3- to 6-fold amplification of the mutated c-Ki-ras. These oncogenic amplifications are accompanied by significantly increased mRNA expression for both genes, suggesting their critical role in tumor progression. PSN-1 was derived through an unconventional process where frozen tumor tissues, stored for 1.5 years at -80°C without cryopreservation agents, were successfully transplanted into nude mice. From the resulting xenograft, the PSN-1 cell line was established and demonstrated stable oncogenic amplifications in vitro, mirroring those found in the original tumor and its metastatic lymph nodes. The cell line displays heterogeneous growth characteristics, with cells either adhering strongly as monolayers or growing in suspension. This dual behavior reflects a diverse cell population capable of adapting to different culture conditions.
Organism	Human
Tissue	Pancreas
Disease	Pancreatic adenocarcinoma
Applications	3D cell culture
Synonyms	PSN1

Age	Unspecified
Gender	Male
Ethnicity	East Asian
Morphology	Epithelial-like
Cell type	Epithelial cell
Growth properties	Adherent

Citation	PSN-1 (Cytion catalog number 305618)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1644

Mutational profile	Gene deletion: CDKN2A, Homozygous; Gene deletion: SMAD4, Homozygous; Mutation: KRAS, Simple, p.Gly12Arg (c.34G>C), Heterozygous; Mutation: TP53, Simple, p.Lys132Gln (c.394A>C), Homozygous

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% heat-inactivated FBS
Dissociation Reagent	Accutase
Doubling time	21 hours
Seeding density	1-3*10^4/cm²
Fluid renewal	2 to 3 times per week
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

PSN-1 Cells

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