PM-LGSOC-01 Cells
General information
Organism | Human |
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Tissue | Ovary |
Disease | Low grade-serous ovarian carcinoma |
Metastatic site | Peritoneum |
Synonyms | M28/2 |
Characteristics
Age | 60 years |
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Gender | Female |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | PM-LGSOC-01 (Cytion catalog number 300305) |
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Biosafety level | 1 |
Depositor | Olivier De Wever |
Expression / Mutation
Mutational profile | KRAS c.35 G > T (p.(Gly12Val)) mutation |
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Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Trypsin/EDTA and Ca2+/Mg2+ free phosphate buffer |
Doubling time | 42 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:20 is recommened |
Seeding density | 1 x 10^4 cells/cm^2 |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 10,11
D13S317: 12,13
D16S539: 10,13
D5S818: 11,12
D7S820: 9,10
TH01: 6,7
TPOX: 8,10
vWA: 15,17
D3S1358: 14,15
D21S11: 28,32
D18S51: 12,17
D8S1179: 13,14
FGA: 23,24
D2S1338: 24,25
D19S433: 12,16
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