PK-15 Cells
Exploring swine fever virus interaction with PK-15 cells
Description | The PK(15) cell line, derived from PK-2A, a cell line obtained from the kidney of an adult pig in 1955, is infected with the porcine type-C oncovirus, also known as the porcine endogenous retrovirus (PERV). The host cell genome contains 62 copies of the pol gene, which codes for reverse transcriptase and other proteins. Initially, the virus particles produced by PK(15) were found to be defective and unable to infect various mammalian cell lines, including a human cell line. However, subsequent studies demonstrated that human 293 cells could be productively infected by the cell-free supernatant from PK(15) cells. Polymerase chain reaction (PCR) analyses showed that the transmitted viruses belonged to the polytropic subtypes PERV-A and PERV-B. Furthermore, it was observed that the virus particles produced by the 293 cells were resistant to inactivation by the human complement system. In addition to its virological significance, the PK(15) cell line also serves specific applications as a suitable transfection host. With its adherent growth properties, the PK(15) cell line proves valuable in various research and experimental settings. |
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Organism | Pig |
Tissue | Kidney |
Synonyms | PK(15), PK (15), PK 15, PK15, Porcine Kidney-15 |
Details of the porcine circovirus cells PK-15
Age | Adult |
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Gender | Male |
Morphology | Epithelial-like |
Growth properties | Monolayer, adherent |
Documentation
Citation | PK-15 (Cytion catalog number 607426) |
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Biosafety level | 2 |
Genomic profile of the PK15 cell line
Viruses | PCV1 (Porcine circovirus 1) positive, PCV2 negative, PCV3 negative |
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Virus susceptibility | Hog cholera, African swine fever, vesicular exanthema of swine, foot and mouth disease (FMDV), vesicular stomatitis (Indiana), vaccinia, reovirus 2, 3, adenovirus 4, 5, coxsackievirus B2, B3, B4, B5, B6 |
Virus resistance | Poliovirus 2 |
Reverse transcriptase | Positive |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:2 to 1:4 is recommended |
Seeding density | 2 x 10^4 cells/cm^2 |
Fluid renewal | 2 to 3 times per week |
Freezing recovery | Allow the cells to recover from the freezing process for at least 24 to 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
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