PA-1 Cells
General information
Description | The line was established from cells taken from ascitic fluid. The cells form tightly knit colonies, and differentiate to form embryoid bodies when cultured in low serum concentration, or at low plating densities or when treated with 5-bromo-2'-deoxyuridine. The embryonic antigen PCC4 is expressed, but F9 is not detectable. |
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Organism | Human |
Tissue | Ovary |
Disease | Ovarian mixed germ cell tumor |
Metastatic site | Ascites |
Synonyms | PA1, PA I, PAI |
Characteristics
Age | 12 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | PA-1 (Cytion catalog number 300402) |
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Biosafety level | 1 |
Depositor | Giovanella |
Expression / Mutation
Antigen expression | HLA A28, B12 |
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Isoenzymes | G6PD, B |
Oncogenes | N-ras (activated) |
Tumorigenic | Yes, in nude mice |
Handling
Culture Medium | EMEM, w: 2 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: EBSS, w: 1 mM Sodium pyruvate, w: NEAA (Cytion article number 820100c) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:10 is recommended |
Fluid renewal | 2 to 3 times per week |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 9,12
D13S317: 9,10
D16S539: 9,9
D5S818: 11
D7S820: 9
TH01: 7,9
TPOX: 11
vWA: 15,17
D3S1358: 15,15
D21S11: 29,31.2
D18S51: 15,18
Penta E: 14,20
Penta D: 9,12
D8S1179: 14,15
FGA: 24,24
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