P3X63Ag8.653 Cells
General information
Description | The cells are resistant to 8-azaguanine and are HAT sensitive. They can be used as fusion partners for producing hybridomas. The cells do not secrete immunoglobulin. The cells have been reported to be cholesterol auxotroph due to a deficiency in 3-ketosteroid reductase activity. |
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Organism | Mouse |
Tissue | Hematopoietic |
Disease | Myeloma |
Synonyms | P3-x63-Ag8.653, P3-x63-Ag8-653, P3-x63-Ag8 653, P3-x63-Ag 8.653, P3-x63Ag8.653, P3-x63.Ag8.653, P3/x63/Ag8.653, P3x63 Ag8.653, P3x63 AG8-653, P3x63-Ag8.653, P3x63-Ag8.653, P3x63 AG 8.653, P3x63Ag8653, P3-x63-Ag8-6-5-3, P3x63Ag8-6-5-3, P3.times.63 Ag8.653, P3.653, x63-Ag 8.6.5.3, x63-AG 8.653, x63-Ag8-653, x63-Ag8.653, x63.Ag8.653, x63Ag8-653, x63Ag8.653, x63AG8.653, P3-653, GM03570, GM3570, GM03570E, NS653 |
Characteristics
Gender | Female |
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Morphology | Round cells |
Growth properties | Adherent/suspension |
Identifiers / Biosafety / Citation
Citation | P3x63Ag8.653 (Cytion catalog number 400118) |
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Biosafety level | 1 |
Expression / Mutation
Viruses | Tested negative for ectromelia virus (mouse pox). |
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Handling
Culture Medium | RPMI 1640, w: 2.1 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Subculturing | Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium. |
Seeding density | Start new cultures at 4 x 10^5 cells/ml. The cell density should not exceed 2 x 10^6 cells/ml. |
Fluid renewal | Every 3 to 4 days. Collect floating cells, centrifuge and add to the flask together with fresh medium. |
Freezing recovery | After thawing, plate the cells at 5 x 10^4 cells/cm^2 and allow the cells to recover from the freezing process and to adhere for at least 48 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
Amelogenin: x,x
M_18-3: 18,19
M_4-2: 21.3
M_6-7: 12
M_3-2: 14,15
M_19-2: 13
M_7-1: 26.2,28.2
M_1-1: 16,17
M_8-1: 13
M_2-1: 15,16
M_15-3: 22.3,23.3
M_6-4: 18,19
M_11-2: 17,18
M_1-2: 17
M_17-2: 16,18
M_12-1: 16
M_5-5: 13,14
M_X-1: 25
M_13-1: 16.2,17.2
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