OE19 Cells

Name: CLS Cell Lines Service GmbH
Address: Dr.-Eckener-Straße 8, Eppelheim, 69214, DE
Telephone: +496221405780
Price range: €€€

USD$375.00*

Products are shipped frozen on dry ice in cryotubes. Each cryotube typically contains 3 × 10⁶ cells for adherent lines or 5 × 10⁶ cells for suspension lines (refer to the batch CoA for details).

Prices excl. taxes plus shipping costs

cryovial

Product number: 305441

Safety data sheet

Product sheet

Certificate of Analysis (CoA)

Description	OE19 is a human oesophageal adenocarcinoma cell line derived from the primary tumor of a patient with Barrett’s oesophagus-associated adenocarcinoma. This cell line is widely utilized in research focused on oesophageal cancers, particularly for investigating tumorigenesis in the context of Barrett’s oesophagus progression. OE19 serves as a model to study the molecular mechanisms underlying adenocarcinoma development, therapeutic responses, and resistance mechanisms in upper gastrointestinal malignancies. OE19 cells exhibit an epithelial morphology and adhere under standard culture conditions. They are characterized by genomic alterations and molecular features typical of oesophageal adenocarcinoma, including overexpression of HER2/neu (ERBB2), a hallmark of aggressive tumor behavior and a clinically significant target for therapy. This makes OE19 particularly relevant for testing HER2-targeted therapies, such as monoclonal antibodies and tyrosine kinase inhibitors. Additionally, OE19 cells are used to explore signaling pathways critical to cancer progression, including MAPK/ERK and PI3K/AKT pathways, as well as mechanisms of immune evasion and interaction with the tumor microenvironment. In preclinical studies, OE19 is valuable for evaluating chemotherapeutic agents, targeted therapies, and novel combinations aimed at overcoming drug resistance. The cell line is also employed in xenograft models to assess tumor growth and therapeutic efficacy in vivo. Its molecular profile and relevance to Barrett’s oesophagus-related adenocarcinoma make OE19 a significant resource for advancing the understanding and treatment of this challenging malignancy.
Organism	Human
Tissue	Esophagus
Disease	Adenocarcinoma
Synonyms	OE-19, JROECL 19, JROECL19, OEC19

Age	72 years
Gender	Male
Ethnicity	European
Morphology	Epithelial-like
Growth properties	Adherent

Citation	OE19 (Cytion catalog number 305441)
Biosafety level	1
NCBI_TaxID	9606
CellosaurusAccession	CVCL_1622

Mutational profile	Mutation: TP53, Simple, p.Asn310Lysfs*27 (c.929dup) (c.929_930ins1), Heterozygous

Culture Medium	RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a)
Supplements	Supplement the medium with 10% FBS
Dissociation Reagent	Accutase 10 min 37°C
Doubling time	50-60 hours
Seeding density	2 to 5 x 10⁴ cells/cm²
Freeze medium	As a cryopreservation medium, we use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress.
Thawing and Culturing Cells	Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks; for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes.
Incubation Atmosphere	37°C, 5% CO₂, humidified atmosphere.
Shipping Conditions	Cryopreserved cell lines are shipped on dry ice in validated, insulated packaging with sufficient refrigerant to maintain approximately −78 °C throughout transit. On receipt, inspect the container immediately and transfer vials without delay to appropriate storage.
Storage Conditions	For long-term preservation, place vials in vapor-phase liquid nitrogen at about −150 to −196 °C. Storage at −80 °C is acceptable only as a short interim step before transfer to liquid nitrogen.

Sterility	Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections.

Lot Number	Certificate Type	Date	Catalog Number
305441-260226	Certificate of Analysis	26. Mar. 2026	305441

OE19 Cells

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Regulatory Data

Biomolecular Data

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Quality Control & Molecular Analysis

Certificate of Analysis (CoA)