OAW-42 Cells
General information
Description | The OAW-42 cell line was established from the ascites of a patient with ovarian cystadenocarcinoma. It has retained the ability to form free floating cysts in vitro, produces extracellular matrix, and shows a defined chemosensitivity pattern. It is a valuable cell line for studies on the biology of human ovarian cancer. |
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Organism | Human |
Tissue | Ovary |
Disease | Cystadenocarcinoma |
Metastatic site | Ascites |
Synonyms | OAW42, OAW 42 |
Characteristics
Age | 46 years |
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Gender | Female |
Ethnicity | Caucasian |
Morphology | Epithelial-like |
Growth properties | Adherent |
Identifiers / Biosafety / Citation
Citation | OAW-42 (Cytion catalog number 300304) |
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Biosafety level | 1 |
Expression / Mutation
Ploidy status | Aneuploid |
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Karyotype | Hypotetraploid |
Handling
Culture Medium | DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 1.5 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) |
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Medium supplements | Supplement the medium with 10% FBS |
Passaging solution | Accutase |
Doubling time | 25 to 30 hours |
Subculturing | Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. |
Split ratio | A ratio of 1:4 to 1:6 is recommended |
Seeding density | 1 x 10^4 cells/cm^2 |
Fluid renewal | 1 to 2 times per week |
Freezing recovery | Fast. Allow the cells to recover from the freezing process for at least 24 hours. |
Freeze medium | CM-1 (Cytion catalog number 800100) or CM-ACF (Cytion catalog number 806100) |
Handling of cryopreserved cultures |
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Quality control / Genetic profile / HLA
Sterility | Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. |
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STR profile |
CSF1PO: 11
D13S317: 11
D16S539: 12,13
D5S818: 11,12
D7S820: 8
TH01: 6,7
TPOX: 8,11
vWA: 15,16
D3S1358: 15,16
D21S11: 26
D18S51: 16,21
Penta E: 12
Penta D: 10
D8S1179: 13
FGA: 22,25
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HLA alleles |
A*: 03:01:01, 30:02:01
B*: 07:02:01, 18:01:01
C*: 05:01:01, 07:02:01
DRB1*: 01:01:01, 03:01:01
DQA1*: 01:01:01, 05:01:01
DQB1*: 02:01:01, 05:01:01
DPB1*: 02:02:01G, 04:02:01G
E: 01:03:02
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